BMRB: 4688

Assignment and secondary structure identification of the ribosomal protein L18 from Thermus thermophilus

Authors

Woestenenk, E.A., Allard, P., Gongadze, G.M., Moskalenko, S.E., Shcherbakov, D.V., Rak, A.V., Garber, M.B., Hard, T., Berglund, H.

Assembly

L18

Entity

1. L18 (polymer, Thiol state: not present), 111 monomers, 12480.42 Da Detail

ARLTAYERRK FRVRNRIKRT GRLRLSVFRS LKHIYAQIID DEKGVTLVSA SSLALKLKGN KTEVARQVGR ALAEKALALG IKQVAFDRGP YKYHGRVKAL AEGAREGGLE F

Formula weight

12480.42 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Refine. method

simulated annealing,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 79.2 %, Completeness (bb): 81.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.2 % (1048 of 1323)	83.8 % (583 of 696)	69.9 % (358 of 512)	93.0 % (107 of 115)
Backbone	81.2 % (539 of 664)	93.5 % (216 of 231)	68.4 % (221 of 323)	92.7 % (102 of 110)
Sidechain	80.0 % (608 of 760)	78.9 % (367 of 465)	81.4 % (236 of 290)	100.0 % (5 of 5)
Aromatic	26.2 % (21 of 80)	52.5 % (21 of 40)	0.0 % (0 of 40)
Methyl	90.7 % (127 of 140)	90.0 % (63 of 70)	91.4 % (64 of 70)

1. L18

ARLTAYERRK FRVRNRIKRT GRLRLSVFRS LKHIYAQIID DEKGVTLVSA SSLALKLKGN KTEVARQVGR ALAEKALALG IKQVAFDRGP YKYHGRVKAL AEGAREGGLE F

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	L18	[U-13C; U-15N]		0.7 ~ 1.6 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 27 models in PDB: 1ILY, Strand ID: A Detail

---

Release date

2000-07-06

Citation

Letter to the editor: assignment and secondary structure identification of the ribosomal protein L18 from Thermus thermophilus
Woestenenk, E.A., Allard, P., Gongadze, G.M., Moskalenko, S.E., Shcherbakov, D.V., Rak, A.V., Garber, M.B., Hard, T., Berglund, H.
J. Biomol. NMR (2000), 17, 273-274, PubMed 10959636 , DOI 10.2210/pdb1emw/pdb , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. L18, : 101 : 249 entities Detail

Experiments performed 11 experiments Detail