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BMRB: 4692


4692
1EL2
SOLUTION STRUCTURE OF A HUMAN TELOMERE FRAGMENT
Authors
Phan, A.T., Gueron, M., Leroy, J.L.
Assembly
TELOMERE FRAGMENT
Entity
1. TELOMERE FRAGMENT (polymer, Thiol state: not present), 22 monomers, 6702.249 Da Detail

XXXTAAXCCT AACCCXAAXX XT


Formula weight
6702.249 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 63.6 %, Completeness: 99.3 %, Completeness (bb): 99.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H31P
All99.3 % (152 of 153)99.3 % (138 of 139)100.0 % (14 of 14)
Suger, PO499.1 % (111 of 112)99.0 % (97 of 98)100.0 % (14 of 14)
Nucleobase100.0 % (41 of 41)100.0 % (41 of 41)
Aromatic100.0 % (28 of 28)100.0 % (28 of 28)
Methyl100.0 % (3 of 3)100.0 % (3 of 3)

1. 5'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'

XXXTAAXCCT AACCCXAAXX XT

Show sample condition
Sample

Pressure 1 atm, Temperature 303 K, pH 5


#NameIsotope labelingTypeConcentration
15'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'1.5 ~ 4.0 mM

Chem. Shift Complete2
Sequence coverage: 4.5 %, Completeness: 50.3 %, Completeness (bb): 49.6 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H31P
All50.3 % (154 of 306)50.4 % (140 of 278)50.0 % (14 of 28)
Suger, PO449.6 % (111 of 224)49.5 % (97 of 196)50.0 % (14 of 28)
Nucleobase52.4 % (43 of 82)52.4 % (43 of 82)
Aromatic53.6 % (30 of 56)53.6 % (30 of 56)
Methyl50.0 % (3 of 6)50.0 % (3 of 6)

1. 5'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'

XXXTAAXCCT AACCCXAAXX XT

Show sample condition
Sample

Pressure 1 atm, Temperature 303 K, pH 5


#NameIsotope labelingTypeConcentration
15'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'1.5 ~ 4.0 mM

Chem. Shift Complete3
Sequence coverage: 4.5 %, Completeness: 34.0 %, Completeness (bb): 33.0 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H31P
All34.0 % (156 of 459)34.1 % (142 of 417)33.3 % (14 of 42)
Suger, PO433.0 % (111 of 336)33.0 % (97 of 294)33.3 % (14 of 42)
Nucleobase36.6 % (45 of 123)36.6 % (45 of 123)
Aromatic38.1 % (32 of 84)38.1 % (32 of 84)
Methyl33.3 % (3 of 9)33.3 % (3 of 9)

1. 5'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'

XXXTAAXCCT AACCCXAAXX XT

Show sample condition
Sample

Pressure 1 atm, Temperature 303 K, pH 5


#NameIsotope labelingTypeConcentration
15'-D(*CP*CP*CP*TP*AP*AP*(5MC)P*CP*CP*TP*AP*AP*CP*CP*CP*UP*AP*AP*CP*CP*CP*T)-3'1.5 ~ 4.0 mM

Release date
2001-03-01
Citation
The solution structure and internal motions of a fragment of the cytidine-rich strand of the human telomere
Phan, A.T., Gueron, M., Leroy, J.L.
J. Mol. Biol. (2000), 299, 123-144, PubMed 10860727 , DOI 10.1006/jmbi.2000.3613 ,
Experiments performed 7 experiments Detail
nullKeywords DNA solution structure, i-motif, interacting loops, loop motions, NMR, telomere