BMRB: 4697

Structure of the fMet-tRNAfMet-binding domain of B. stearothermophilus initiation factor IF2

Authors

Meunier, S., Spurio, R., Czisch, M., Wechselberger, R., Guennuegues, M., Gualerzi, C.O., Boelens, R.

Assembly

fMet-tRNA binding domain of initiation factor IF2

Entity

1. fMet-tRNA binding domain of initiation factor IF2 (polymer, Thiol state: not reported), 112 monomers, 12610.24 Da Detail

EIEAAMKGML DPEYEEKVIG QAEVRQTFKV SKVGTIAGCY VTDGKITRDS KVRLIRQGIV VYEGEIDSLK RYKDDVREVA QGYECGLTIK NFNDIKEGDV IEAYVMQEVA RA

Formula weight

12610.24 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 80.4 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.4 % (1043 of 1297)	88.6 % (599 of 676)	65.2 % (328 of 503)	98.3 % (116 of 118)
Backbone	93.0 % (623 of 670)	96.1 % (224 of 233)	88.7 % (289 of 326)	99.1 % (110 of 111)
Sidechain	67.8 % (494 of 729)	84.7 % (375 of 443)	40.5 % (113 of 279)	85.7 % (6 of 7)
Aromatic	48.5 % (33 of 68)	85.3 % (29 of 34)	11.8 % (4 of 34)
Methyl	57.5 % (77 of 134)	82.1 % (55 of 67)	32.8 % (22 of 67)

1. fMet-tRNA binding domain of initiation factor IF2

EIEAAMKGML DPEYEEKVIG QAEVRQTFKV SKVGTIAGCY VTDGKITRDS KVRLIRQGIV VYEGEIDSLK RYKDDVREVA QGYECGLTIK NFNDIKEGDV IEAYVMQEVA RA

Show sample condition

Sample

Temperature 312 (±1) K, pH 5.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	fMet-tRNA binding domain of initiation factor IF2	[U-98% 15N]		1.5 mM

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LACS Plot; CA

Referencing offset: -0.6 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.6 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.06 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1D1N, Strand ID: A Detail

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Heteronucl. T₁

90 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 0.0 MHz, Temperature 312 (±1) K, pH 5.2 (±0.1) Detail

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Temperature 312 (±1) K, pH 5.2 (±0.1)

Heteronucl. T₂

90 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 0.0 MHz, Temperature 312 (±1) K, pH 5.2 (±0.1) Detail

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Temperature 312 (±1) K, pH 5.2 (±0.1)

Heteronucl. NOE

90 NOE values in 1 lists
Value type na, Field strength (¹H) 0.0 MHz, Temperature 312 (±1) K, pH 5.2 (±0.1) Detail

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Temperature 312 (±1) K, pH 5.2 (±0.1)

Heteronucl. T₁/T₂

90 T₁/T₂ values in 1 lists
Field strength (¹H) 0.0 MHz, Temperature 312 (±1) K, pH 5.2 (±0.1) Detail

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Temperature 312 (±1) K, pH 5.2 (±0.1)

Release date

2001-07-08

Citation

Structure of the fMet-tRNA(fMet)-binding domain of B. stearothermophilus initiation factor IF2
Meunier, S., Spurio, R., Czisch, M., Wechselberger, R., Guennuegues, M., Gualerzi, C.O., Boelens, R.
EMBO J. (2000), 19, 1918-1926, PubMed 10775275 , DOI 10.1093/emboj/19.8.1918 , Abstract

Related entities 1. fMet-tRNA binding domain of initiation factor IF2, : 1 : 1 : 1 : 126 entities Detail

Experiments performed 1 experiments Detail