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Found 1 Document (1.643 seconds)

BMRB: 4701

1D9S

Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data

Authors

Yuan, C., Selby, T.L., Byeon, I.L., Tsai, M.

Assembly

mouse p15(INK4B) tumor suppressor

Entity

1. mouse p15(INK4B) tumor suppressor (polymer, Thiol state: all free), 136 monomers, 14337.05 Da Detail

GSPGIHMLGG SSDAGLATAA ARGQVETVRQ LLEAGADPNA LNRFGRRPIQ VMMMGSAQVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVCDAW GRLPVDLAEE QGHRDIARYL HAATGD

Formula weight

14337.05 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

COMPARATIVE MODELING, RESTRAINT ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 68.7 %, Completeness (bb): 73.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.7 % (992 of 1445)	79.9 % (592 of 741)	49.3 % (279 of 566)	87.7 % (121 of 138)
Backbone	73.7 % (591 of 802)	89.6 % (251 of 280)	56.2 % (221 of 393)	92.2 % (119 of 129)
Sidechain	63.9 % (488 of 764)	74.0 % (341 of 461)	49.3 % (145 of 294)	22.2 % (2 of 9)
Aromatic	31.3 % (20 of 64)	62.5 % (20 of 32)	0.0 % (0 of 31)	0.0 % (0 of 1)
Methyl	80.8 % (139 of 172)	91.9 % (79 of 86)	69.8 % (60 of 86)

1. p15

GSPGIHMLGG SSDAGLATAA ARGQVETVRQ LLEAGADPNA LNRFGRRPIQ VMMMGSAQVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVCDAW GRLPVDLAEE QGHRDIARYL HAATGD

Show sample condition

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1D9S, Strand ID: A Detail

Release date

2000-05-08

Citation

Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data
Yuan, C., Selby, T.L., Li, J., Byeon, I.L., Tsai, M.
Protein Sci. (2000), PubMed , DOI:

Related entities 1. mouse p15(INK4B) tumor suppressor, : 1 : 2 : 560 entities Detail

Interaction partners 1. mouse p15(INK4B) tumor suppressor, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 5 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

2 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

3 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

4 1H-15N NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

5 1H-13C NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	p15	[U-15N]	0.2 ~ 0.4 mM
2	HEPES		4 mM
3	DTT		1 mM
4	EDTA		5. uM
5	D2O		5 %
6	H2O		95 %

Sample #2

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
7	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

Sample #3

Temperature 293 (±1) K, pH 7.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
8	p15	[U-15N; U-13C]		0.2 ~ 0.4 mM

nullKeywords p15INK4B

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Found 1 Document (1.643 seconds)

BMRB: 4701

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. mouse p15(INK4B) tumor suppressor, : 1 : 2 : 560 entities Detail

Interaction partners 1. mouse p15(INK4B) tumor suppressor, : 1 interactors Detail

Experiments performed 5 experiments Detail

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