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Found 1 Document (1.852 seconds)

BMRB: 4706

1CX1

Structure and Binding Specificity of the Second N-Terminal Cellulose-Binding Domain from Cellulomonas fimi Endoglucanase C

Authors

Brun, E., Johnson, P.E., Creagh, L.A., Haynes, C.A., Tomme, P., Webster, P., McIntosh, L.P.

Assembly

CELLULOSE BINDING DOMAIN N2 FROM C. FIMI ENDOGLUCANASE C

Entity

1. CELLULOSE BINDING DOMAIN N2 FROM C. FIMI ENDOGLUCANASE C (polymer, Thiol state: all disulfide bound), 153 monomers, 15870.30 Da Detail

ASLDSEVELL PHTSFAESLG PWSLYGTSEP VFADGRMCVD LPGGQGNPWD AGLVYNGVPV GEGESYVLSF TASATPDMPV RVLVGEGGGA YRTAFEQGSA PLTGEPATRE YAFTSNLTFP PDGDAPGQVA FHLGKAGAYE FCISQVSLTT SAT

Formula weight

15870.3 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS38:SG	1:CYS142:SG

Source organism

Cellulomonas fimi

Exptl. method

NMR

Refine. method

ALL CALCULATIONS WERE PERFORMED USING X-PLOR V3.8 WITH SCRIPTS FROM THE X-PLOR 3.1 MANUAL A. A. BRUNGER (1992), NEW HAVEN: YALE UNIVERSITY PRESS ACCORDING TO THE METHOD DESCRIBED BY M. NILGES, G. M. CLORE & A. M. GRONENBORN

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.3 %, Completeness (bb): 85.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.3 % (1467 of 1625)	97.6 % (805 of 825)	79.0 % (514 of 651)	99.3 % (148 of 149)
Backbone	85.7 % (764 of 892)	99.0 % (310 of 313)	71.8 % (315 of 439)	99.3 % (139 of 140)
Sidechain	96.5 % (836 of 866)	96.7 % (495 of 512)	96.2 % (332 of 345)	100.0 % (9 of 9)
Aromatic	84.4 % (135 of 160)	85.0 % (68 of 80)	83.3 % (65 of 78)	100.0 % (2 of 2)
Methyl	98.8 % (158 of 160)	97.5 % (78 of 80)	100.0 % (80 of 80)

1. ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2

ASLDSEVELL PHTSFAESLG PWSLYGTSEP VFADGRMCVD LPGGQGNPWD AGLVYNGVPV GEGESYVLSF TASATPDMPV RVLVGEGGGA YRTAFEQGSA PLTGEPATRE YAFTSNLTFP PDGDAPGQVA FHLGKAGAYE FCISQVSLTT SAT

Show sample condition

Sample

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbons	0.0 ppm	external	direct
¹H	DSS	methyl protons	0.0 ppm	external	direct
¹⁵N	ammonium chloride	nitrogen	24.93 ppm	external	direct

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbons	0.0 ppm	external	direct
¹H	DSS	methyl protons	0.0 ppm	external	direct
¹⁵N	ammonium chloride	nitrogen	24.93 ppm	external	direct

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbons	0.0 ppm	external	direct
¹H	DSS	methyl protons	0.0 ppm	external	direct
¹⁵N	ammonium chloride	nitrogen	24.93 ppm	external	direct

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 1CX1, Strand ID: A Detail

Release date

2000-05-25

Citation

Structure and binding specificity of the second N-terminal cellulose-binding domain from Cellulomonas fimi endoglucanase C
Brun, E., Johnson, P.E., Creagh, A.L., Haynes, C.A., Tomme, P., Webster, P., McIntosh, L.P.
Biochemistry (2000), 39, 2445-2458, PubMed 10704194 , DOI: , Abstract

Related entities 1. CELLULOSE BINDING DOMAIN N2 FROM C. FIMI ENDOGLUCANASE C, : 1 : 10 entities Detail

Experiments performed 5 experiments Detail

1 3D 13C-SEPARATED_NOESY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
5	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N]	1.5 ~ 2.0 mM
6	Cellopentaose		44 mM
7	NaCl		50 mM
8	PHOSPHATE BUFFER		50 mM

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Concentration
9	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	1.5 ~ 2.0 mM
10	Cellopentaose	44 mM
11	NaCl	50 mM
12	PHOSPHATE BUFFER	50 mM

Sample #4

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
13	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	non-random 10% 13C		1.5 ~ 2.0 mM

2 3D 15N-SEPARATED_NOESY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
5	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N]	1.5 ~ 2.0 mM
6	Cellopentaose		44 mM
7	NaCl		50 mM
8	PHOSPHATE BUFFER		50 mM

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Concentration
9	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	1.5 ~ 2.0 mM
10	Cellopentaose	44 mM
11	NaCl	50 mM
12	PHOSPHATE BUFFER	50 mM

Sample #4

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
13	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	non-random 10% 13C		1.5 ~ 2.0 mM

3 2D NOESY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
5	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N]	1.5 ~ 2.0 mM
6	Cellopentaose		44 mM
7	NaCl		50 mM
8	PHOSPHATE BUFFER		50 mM

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Concentration
9	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	1.5 ~ 2.0 mM
10	Cellopentaose	44 mM
11	NaCl	50 mM
12	PHOSPHATE BUFFER	50 mM

Sample #4

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
13	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	non-random 10% 13C		1.5 ~ 2.0 mM

4 4D 13C/15N-SEPARATED_NOESY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
5	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N]	1.5 ~ 2.0 mM
6	Cellopentaose		44 mM
7	NaCl		50 mM
8	PHOSPHATE BUFFER		50 mM

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Concentration
9	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	1.5 ~ 2.0 mM
10	Cellopentaose	44 mM
11	NaCl	50 mM
12	PHOSPHATE BUFFER	50 mM

Sample #4

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
13	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	non-random 10% 13C		1.5 ~ 2.0 mM

5 HNHA

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
1	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N; U-99% 13C]	1.5 ~ 2.0 mM
2	Cellopentaose		44 mM
3	NaCl		50 mM
4	PHOSPHATE BUFFER		50 mM

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Concentration
5	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	[U-99% 15N]	1.5 ~ 2.0 mM
6	Cellopentaose		44 mM
7	NaCl		50 mM
8	PHOSPHATE BUFFER		50 mM

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Concentration
9	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	1.5 ~ 2.0 mM
10	Cellopentaose	44 mM
11	NaCl	50 mM
12	PHOSPHATE BUFFER	50 mM

Sample #4

Pressure 1 atm, Temperature 308 (±1) K, pH 6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
13	ENDOGLUCANASE C CELLULOE BINDING DOMAIN N2	non-random 10% 13C		1.5 ~ 2.0 mM

nullKeywords CELLOOLIGOSACHARIDES, CELLULASE, CELLULOSE-BINDING DOMAIN, NMR, PROTEIN- CARBOHYDRATE INTERACTION

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Found 1 Document (1.852 seconds)

BMRB: 4706

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CELLULOSE BINDING DOMAIN N2 FROM C. FIMI ENDOGLUCANASE C, : 1 : 10 entities Detail

Experiments performed 5 experiments Detail

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