BMRB: 4716

1H, 15N, and 13C NMR Backbone Assignments and Secondary Structure of the C-terminal Recombinant Fragment of Auxilin Including the J-domain

Authors

Han, C.J., Gruschus, J.M., Greener, T., Greene, L.E., Ferretti, J., Eisenberg, E.

Assembly

auxilin

Entity

1. auxilin (polymer, Thiol state: not present), 182 monomers, 20563.19 Da Detail

GPLGSPEFSM PHSSPQNRPN YNVSFSSMPG GQNERGKAAA NLEGKQKAAD FEDLLSGQGF NAHKDKKGPR TIAEMRKEEM AKEMDPEKLK ILEWIEGKER NIRALLSTMH TVLWAGETKW KPVGMADLVT PEQVKKVYRK AVLVVHPDKA TGQPYEQYAK MIFMELNDAW SEFENQGQKP LY

Formula weight

20563.19 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 53.3 %, Completeness (bb): 74.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	53.3 % (1156 of 2170)	52.4 % (602 of 1149)	47.8 % (397 of 830)	82.2 % (157 of 191)
Backbone	74.6 % (795 of 1066)	81.4 % (297 of 365)	64.8 % (345 of 532)	90.5 % (153 of 169)
Sidechain	40.7 % (518 of 1272)	38.9 % (305 of 784)	44.8 % (209 of 466)	18.2 % (4 of 22)
Aromatic	52.4 % (86 of 164)	52.4 % (43 of 82)	50.0 % (39 of 78)	100.0 % (4 of 4)
Methyl	43.3 % (65 of 150)	61.3 % (46 of 75)	25.3 % (19 of 75)

1. auxilin

GPLGSPEFSM PHSSPQNRPN YNVSFSSMPG GQNERGKAAA NLEGKQKAAD FEDLLSGQGF NAHKDKKGPR TIAEMRKEEM AKEMDPEKLK ILEWIEGKER NIRALLSTMH TVLWAGETKW KPVGMADLVT PEQVKKVYRK AVLVVHPDKA TGQPYEQYAK MIFMELNDAW SEFENQGQKP LY

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	auxilin			1.0 ~ 2.0 mM

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1N4C, Strand ID: A Detail

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Release date

2000-07-23

Citation

Letter to the Editor: 1H, 15N, and 13C NMR backbone assignments and secondary structure of the C-terminal recombinant fragment of auxilin including the J-domain
Han, C.J., Gruschus, J.M., Greener, T., Greene, L.E., Ferretti, J., Eisenberg, E.
J. Biomol. NMR (2000), 17, 281-282, PubMed , DOI:

Related entities 1. auxilin, : 1 : 16 entities Detail

Interaction partners 1. auxilin, : 3 interactors Detail

Experiments performed 4 experiments Detail