BMRB: 4717

1H, 15N, 13C, and 13CO Assignments for ZipA

Authors

Moy, F.J., Glasfeld, E., Powers, R.

Assembly

C-terminal FtsZ binding domain of E. coli ZipA

Entity

1. C-terminal FtsZ binding domain of E. coli ZipA (polymer, Thiol state: not present), 144 monomers, 16116.26 Da Detail

MDKPKRKEAV IIMNVAAHHG SELNGELLLN SIQQAGFIFG DMNIYHRHLS PDGSGPALFS LANMVKPGTF DPEMKDFTTP GVTIFMQVPS YGDELQNFKL MLQSAQHIAD EVGGVVLDDQ RRMMTPQKLR EYQDIIREVK DANA

Formula weight

16116.26 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.1 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.1 % (1498 of 1682)	87.4 % (768 of 879)	92.0 % (599 of 651)	86.2 % (131 of 152)
Backbone	96.1 % (815 of 848)	96.9 % (282 of 291)	95.7 % (403 of 421)	95.6 % (130 of 136)
Sidechain	83.0 % (803 of 967)	81.1 % (477 of 588)	89.5 % (325 of 363)	6.3 % (1 of 16)
Aromatic	57.9 % (66 of 114)	61.4 % (35 of 57)	54.4 % (31 of 57)
Methyl	96.0 % (144 of 150)	96.0 % (72 of 75)	96.0 % (72 of 75)

1. E. coli ZipA

MDKPKRKEAV IIMNVAAHHG SELNGELLLN SIQQAGFIFG DMNIYHRHLS PDGSGPALFS LANMVKPGTF DPEMKDFTTP GVTIFMQVPS YGDELQNFKL MLQSAQHIAD EVGGVVLDDQ RRMMTPQKLR EYQDIIREVK DANA

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	E. coli ZipA	[U-15N]		1.0 mM
2	potassium phosphate			50 mM
3	NaN3			2 mM
4	DTT	[U-2H]		50 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	E. coli ZipA	[U-13C; U-15N]		1.0 mM
8	potassium phosphate			50 mM
9	NaN3			2 mM
10	DTT	[U-2H]		50 mM
11	H2O			90 %
12	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1F7W, Strand ID: A Detail

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Release date

2000-05-23

Citation

Letter to the Editor: 1H, 15N, 13C, and 13CO assignments and secondary structure determination of ZipA*
Moy, F.J., Glasfeld, E., Powers, R.
J. Biomol. NMR (2000), 17, 275-276, PubMed , DOI:

Related entities 1. C-terminal FtsZ binding domain of E. coli ZipA, : 1 : 14 : 7 : 85 entities Detail

Interaction partners 1. C-terminal FtsZ binding domain of E. coli ZipA, : 2 interactors Detail