BMRBj - BMREntryMaster

Found 1 Document (1.813 seconds)

BMRB: 4721

1D8J

Structure of the Central Core Domain of TFIIEbeta with a Novel Double-stranded DNA-binding Surface

Authors

Okuda, M., Watanabe, Y., Okamura, H., Hanaoka, F., Ohkuma, Y., Nishimura, Y.

Assembly

general transcription factor TFIIE-BETA

Entity

1. general transcription factor TFIIE-BETA (polymer, Thiol state: not present), 81 monomers, 9272.576 Da Detail

ALSGSSGYKF GVLAKIVNYM KTRHQRGDTH PLTLDEILDE TQHLDIGLKQ KQWLMTEALV NNPKIEVIDG KYAFKPKYNV R

Formula weight

9272.576 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

4D SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.5 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (861 of 984)	98.3 % (506 of 515)	70.4 % (269 of 382)	98.9 % (86 of 87)
Backbone	84.6 % (406 of 480)	98.2 % (162 of 165)	70.5 % (167 of 237)	98.7 % (77 of 78)
Sidechain	91.5 % (530 of 579)	98.3 % (344 of 350)	80.5 % (177 of 220)	100.0 % (9 of 9)
Aromatic	51.3 % (39 of 76)	100.0 % (38 of 38)	0.0 % (0 of 37)	100.0 % (1 of 1)
Methyl	97.9 % (92 of 94)	100.0 % (47 of 47)	95.7 % (45 of 47)

1. general transcription factor TFIIE-BETA

ALSGSSGYKF GVLAKIVNYM KTRHQRGDTH PLTLDEILDE TQHLDIGLKQ KQWLMTEALV NNPKIEVIDG KYAFKPKYNV R

Show sample condition

Sample

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1D8J, Strand ID: A Detail

Release date

2000-12-17

Citation

Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface
Okuda, M., Watanabe, Y., Okamura, H., Hanaoka, F., Ohkuma, Y., Nishimura, Y.
EMBO J. (2000), 19, 1346-1356, PubMed 10716934 , DOI 10.1093/emboj/19.6.1346 , Abstract

Related entities 1. general transcription factor TFIIE-BETA, : 1 : 11 : 5 : 5 entities Detail

Interaction partners 1. general transcription factor TFIIE-BETA, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

2 3D_13C-SEPARATED_NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

3 3D_15N-SEPARATED_NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

4 4D_13C-SEPARATED_NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

5 HNHA

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

6 HMQC-J

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	general transcription factor TFIIE-BETA	[U-15N; U-13C]	1.0 ~ 2.0 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM

Sample #2

Temperature 300 K, pH 6.0

#	Name	Concentration
4	general transcription factor TFIIE-BETA	1.0 ~ 2.0 mM
5	phosphate buffer	20 mM
6	NaCl	500 mM

nullKeywords WINGED HELIX-TURN-HELIX

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.813 seconds)

BMRB: 4721

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. general transcription factor TFIIE-BETA, : 1 : 11 : 5 : 5 entities Detail

Interaction partners 1. general transcription factor TFIIE-BETA, : 9 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2