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Found 1 Document (2.306 seconds)

BMRB: 4735

1JYT

Sequence-specific NMR resonance assignments for the backbone atoms of olfactory marker protein (OMP)

Authors

Baldisseri, D.M., Margolis, J.W., Omotosho, P.A., Volkman, B.F., Margolis, F.L.

Assembly

olfactory marker protein

Entity

1. olfactory marker protein (polymer, Thiol state: not present), 163 monomers, 18852.32 Da Detail

MAEDGPQKQQ LDMPLVLDQD LTKQMRLRVE SLKQRGEKKQ DGEKLLRPAE SVYRLDFIQQ QKLQFDHWNV VLDKPGKVTI TGTSQNWTPD LTNLMTRQLL DPAAIFWRKE DSDAMDWNEA DALEFGERLS DLAKIRKVMY FLITFGEGVE PANLKASVVF NQL

Formula weight

18852.32 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 48.7 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.7 % (965 of 1983)	33.2 % (346 of 1043)	61.4 % (467 of 760)	84.4 % (152 of 180)
Backbone	96.2 % (927 of 964)	97.6 % (319 of 327)	94.8 % (456 of 481)	97.4 % (152 of 156)
Sidechain	15.9 % (187 of 1174)	3.8 % (27 of 716)	36.9 % (160 of 434)	0.0 % (0 of 24)
Aromatic	0.0 % (0 of 138)	0.0 % (0 of 69)	0.0 % (0 of 65)	0.0 % (0 of 4)
Methyl	12.8 % (23 of 180)	6.7 % (6 of 90)	18.9 % (17 of 90)

1. olfactory marker protein

MAEDGPQKQQ LDMPLVLDQD LTKQMRLRVE SLKQRGEKKQ DGEKLLRPAE SVYRLDFIQQ QKLQFDHWNV VLDKPGKVTI TGTSQNWTPD LTNLMTRQLL DPAAIFWRKE DSDAMDWNEA DALEFGERLS DLAKIRKVMY FLITFGEGVE PANLKASVVF NQL

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JYT, Strand ID: A Detail

Release date

2000-09-24

Citation

Letter to the Editor: Sequence-specific NMR resonance assignments of the backbone atoms for the olfactory marker protein, OMP
Baldisseri, D.M., Margolis, J.W., Omotosho, P.A., Volkman, B.F., Margolis, F.L.
J. Biomol. NMR (2000), 17, 353-354, PubMed , DOI:

Related entities 1. olfactory marker protein, : 1 : 3 : 2 : 7 entities Detail

Interaction partners 1. olfactory marker protein, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

2 3D CBCA(CO)NH

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

3 3D HNCACB

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

4 3D HNCO

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

5 3D HCACO

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

6 3D HCA(CO)N

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

7 3D HNHA

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

8 3D 15N-NOESY-HSQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

9 3D 15N,15N-HMQC-NOESY-HSQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

10 4D 15N,13C-NOESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	olfactory marker protein	[U-15N]		1.5 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	olfactory marker protein	[U-15N;U-13C]		1.8 mM

null

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Found 1 Document (2.306 seconds)

BMRB: 4735

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. olfactory marker protein, : 1 : 3 : 2 : 7 entities Detail

Interaction partners 1. olfactory marker protein, : 3 interactors Detail

Experiments performed 10 experiments Detail

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