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Found 1 Document (3.592 seconds)

BMRB: 4740

1GH9

Solution Structure of a 8.3 kDa Protein (gene MTH1184) from Methanobacterium thermoautotrophicum

Authors

Kozlov, G., Ekiel, I., Gehring, K.

Assembly

MTH1184 monomer

Entity

1. MTH1184 monomer (polymer, Thiol state: all free), 71 monomers, 8325.424 Da Detail

MYIIFRCDCG RALYSREGAK TRKCVCGRTV NVKDRRIFGR ADDFEEASEL VRKLQEEKYG SCHFTNPSKR E

Formula weight

8325.424 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 58.5 %, Completeness (bb): 75.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	58.5 % (491 of 839)	64.9 % (289 of 445)	41.7 % (134 of 321)	93.2 % (68 of 73)
Backbone	75.2 % (319 of 424)	96.6 % (141 of 146)	52.9 % (110 of 208)	97.1 % (68 of 70)
Sidechain	44.7 % (215 of 481)	49.5 % (148 of 299)	37.4 % (67 of 179)	0.0 % (0 of 3)
Aromatic	8.8 % (6 of 68)	17.6 % (6 of 34)	0.0 % (0 of 34)
Methyl	87.0 % (47 of 54)	88.9 % (24 of 27)	85.2 % (23 of 27)

1. 8.3 KDA PROTEIN (GENE MTH1184)

MYIIFRCDCG RALYSREGAK TRKCVCGRTV NVKDRRIFGR ADDFEEASEL VRKLQEEKYG SCHFTNPSKR E

Show sample condition

Sample #1

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM

Sample #2

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
5	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C]	1.0 ~ 2.0 mM
6	phosphate buffer		50 mM
7	NaCl		0.15 M
8	DTT		1 mM

Sample #3

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
9	8.3 KDA PROTEIN (GENE MTH1184)	[U-15N]	1.0 ~ 2.0 mM
10	phosphate buffer		50 mM
11	NaCl		0.15 M
12	DTT		1 mM

Sample #4

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Concentration
13	8.3 KDA PROTEIN (GENE MTH1184)	1.0 ~ 2.0 mM
14	phosphate buffer	50 mM
15	NaCl	0.15 M
16	DTT	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1GH9, Strand ID: A Detail

Coupling constant

60 J values in 1 lists
Temperature 305 (±0.1) K, pH 6.3 (±0.1) Detail

Release date

2002-03-31

Citation

Structural proteomics of an archaeon
Christendat, D., Yee, A., Dharamsi, A., Kluger, Y., Savchenko, A., Cort, J.R., Booth, V., Mackereth, C.D., Saridakis, V., Ekiel, I., Kozlov, G., Maxwell, K.L., Wu, N., Mcintosh, L.P., Gehring, K., Kennedy, M.A., Davidson, A.R., Pai, E.F., Gerstein, M., Edwards, A.M., Arrowsmith, C.H.
Nat. Struct. Biol. (2000), 7, 903-909, PubMed 11017201 , DOI 10.1038/82823 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 4 entries Detail

  BMRB: 5629 released on 2003-04-07
    Title 1H, 13C, and 15N resonance assignments and topology of MTH187, a conserved protein from Methanobacterium thermoautotrophicum
  BMRB: 4674 released on 2002-09-22
    Title Structural Proteomics of M. thermoautotrophicum: A global survey of non-membrane protein expression, solubility and structure
  Swiss-Prot: O26255 released on 2001-02-21
    Title P152_METTH Entity Protein MTH_152
  Swiss-Prot: O27252 released on 2001-01-24
    Title PA84_METTH Entity Protein MTH_1184
  Swiss-Prot: O26253 released on 2000-12-01
    Title NADM_METTH Entity Nicotinamide-nucleotide adenylyltransferase
  Swiss-Prot: O27652 released on 2000-05-30
    Title DNBP_METTH Entity DNA-binding protein MTH_1615

Related entities 1. MTH1184 monomer, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail

1 3D 13C-SEPARATED NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM

Sample #2

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
5	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C]	1.0 ~ 2.0 mM
6	phosphate buffer		50 mM
7	NaCl		0.15 M
8	DTT		1 mM

Sample #3

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
9	8.3 KDA PROTEIN (GENE MTH1184)	[U-15N]	1.0 ~ 2.0 mM
10	phosphate buffer		50 mM
11	NaCl		0.15 M
12	DTT		1 mM

Sample #4

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Concentration
13	8.3 KDA PROTEIN (GENE MTH1184)	1.0 ~ 2.0 mM
14	phosphate buffer	50 mM
15	NaCl	0.15 M
16	DTT	1 mM

2 3D 15N-SEPARATED NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM

Sample #2

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
5	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C]	1.0 ~ 2.0 mM
6	phosphate buffer		50 mM
7	NaCl		0.15 M
8	DTT		1 mM

Sample #3

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
9	8.3 KDA PROTEIN (GENE MTH1184)	[U-15N]	1.0 ~ 2.0 mM
10	phosphate buffer		50 mM
11	NaCl		0.15 M
12	DTT		1 mM

Sample #4

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Concentration
13	8.3 KDA PROTEIN (GENE MTH1184)	1.0 ~ 2.0 mM
14	phosphate buffer	50 mM
15	NaCl	0.15 M
16	DTT	1 mM

3 HNHA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM

Sample #2

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
5	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C]	1.0 ~ 2.0 mM
6	phosphate buffer		50 mM
7	NaCl		0.15 M
8	DTT		1 mM

Sample #3

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
9	8.3 KDA PROTEIN (GENE MTH1184)	[U-15N]	1.0 ~ 2.0 mM
10	phosphate buffer		50 mM
11	NaCl		0.15 M
12	DTT		1 mM

Sample #4

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Concentration
13	8.3 KDA PROTEIN (GENE MTH1184)	1.0 ~ 2.0 mM
14	phosphate buffer	50 mM
15	NaCl	0.15 M
16	DTT	1 mM

4 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM

Sample #2

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
5	8.3 KDA PROTEIN (GENE MTH1184)	[U-13C]	1.0 ~ 2.0 mM
6	phosphate buffer		50 mM
7	NaCl		0.15 M
8	DTT		1 mM

Sample #3

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
9	8.3 KDA PROTEIN (GENE MTH1184)	[U-15N]	1.0 ~ 2.0 mM
10	phosphate buffer		50 mM
11	NaCl		0.15 M
12	DTT		1 mM

Sample #4

Temperature 305 (±0.1) K, pH 6.3 (±0.1)

#	Name	Concentration
13	8.3 KDA PROTEIN (GENE MTH1184)	1.0 ~ 2.0 mM
14	phosphate buffer	50 mM
15	NaCl	0.15 M
16	DTT	1 mM

nullKeywords BETA+ALPHA COMPLEX STRUCTURE

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Found 1 Document (3.592 seconds)

BMRB: 4740

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2, Swiss-Prot: 4 entries Detail

Related entities 1. MTH1184 monomer, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail

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