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BMRB: 4743


4743
1EHJ
1H Chemical Shift Assignments for fully reduced cytochrome c7 from Desulfuromonas acetoxidan
Authors
Assfalg, M., Banci, L., Bertini, I., Bruschi, M., Giudici-Orticoni, M.T., Turano, P.
Assembly
CYTOCHROME C7
Entity
1. CYTOCHROME C7 (polymer, Thiol state: all other bound), 68 monomers, 7262.162 Da Detail

ADVVTYENKK GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIK


2. HEC (non-polymer), 618.503 × 3 Da
Total weight
9117.671 Da
Max. entity weight
7262.162 Da
Source organism
Desulfuromonas acetoxidans
Exptl. method
NMR
Refine. method
simulated annealing combined with torsion angle dynamics restrained energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 97.1 %, Completeness: 89.9 %, Completeness (bb): 95.0 % Detail
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Polymer type: polypeptide(L)

Total1H
All89.9 % (347 of 386)89.9 % (347 of 386)
Backbone95.0 % (133 of 140)95.0 % (133 of 140)
Sidechain87.0 % (214 of 246)87.0 % (214 of 246)
Aromatic52.4 % (11 of 21)52.4 % (11 of 21)
Methyl92.6 % (25 of 27)92.6 % (25 of 27)

1. CYTOCHROME C7

ADVVTYENKK GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIK

Show sample condition
Sample

Temperature 292 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
3CYTOCHROME C72.0 ~ 3.0 mM

Chem. Shift Complete2
Sequence coverage: 4.4 %, Completeness: 47.9 %, Completeness (bb): 49.6 % Detail
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Polymer type: polypeptide(L)

Total1H
All47.9 % (370 of 772)47.9 % (370 of 772)
Backbone49.6 % (139 of 280)49.6 % (139 of 280)
Sidechain47.0 % (231 of 492)47.0 % (231 of 492)
Aromatic38.1 % (16 of 42)38.1 % (16 of 42)
Methyl48.1 % (26 of 54)48.1 % (26 of 54)

1. CYTOCHROME C7

ADVVTYENKK GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIK

Show sample condition
Sample

Temperature 292 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
3CYTOCHROME C72.0 ~ 3.0 mM

Chem. Shift Complete3
Sequence coverage: 1.5 %, Completeness: 32.0 %, Completeness (bb): 33.3 % Detail
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Polymer type: polypeptide(L)

Total1H
All32.0 % (371 of 1158)32.0 % (371 of 1158)
Backbone33.3 % (140 of 420)33.3 % (140 of 420)
Sidechain31.3 % (231 of 738)31.3 % (231 of 738)
Aromatic25.4 % (16 of 63)25.4 % (16 of 63)
Methyl32.1 % (26 of 81)32.1 % (26 of 81)

1. CYTOCHROME C7

ADVVTYENKK GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIK

Show sample condition
Sample

Temperature 292 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
3CYTOCHROME C72.0 ~ 3.0 mM

Chem. Shift Complete4
Sequence coverage: 4.4 %, Completeness: 24.8 %, Completeness (bb): 25.7 % Detail
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Polymer type: polypeptide(L)

Total1H
All24.8 % (383 of 1544)24.8 % (383 of 1544)
Backbone25.7 % (144 of 560)25.7 % (144 of 560)
Sidechain24.3 % (239 of 984)24.3 % (239 of 984)
Aromatic19.0 % (16 of 84)19.0 % (16 of 84)
Methyl24.1 % (26 of 108)24.1 % (26 of 108)

1. CYTOCHROME C7

ADVVTYENKK GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIK

Show sample condition
Sample

Temperature 292 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
3CYTOCHROME C72.0 ~ 3.0 mM

Protein Blocks Logo
Calculated from 1 models in PDB: 1EHJ, Strand ID: A Detail

Release date
2000-07-12
Citation
A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas acetoxidans. Comparison between the reduced and the oxidized forms
Assfalg, M., Banci, L., Bertini, I., Bruschi, M., Giudici-Orticoni, M.T., Turano, P.
Eur. J. Biochem. (1999), 266, 634-643, PubMed 10561607 , DOI: ,
Related entities 1. CYTOCHROME C7, : 1 : 5 : 2 : 30 entities Detail
More details for 38 related entities
Experiments performed 2 experiments Detail
nullKeywords cyt c7, multi-heme