BMRB: 4751

Assignment of 1H and 15N resonances of mouse lysozyme

Authors

Obita, T., Ueda, T., Tanaka, Y., Hashimoto, Y., Imoto, T.

Assembly

mouse lysozyme

Entity

1. mouse lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14820.48 Da Detail

KVYERCEFAR TLKRNGMAGY YGVSLADWVC LAQHESNYNT RATNYNRGDQ STDYGIFQIN SRYWCNDGKT PRAVNACGIN CSALLQDDIT AAIQCAKRVV RDPQGIRAWV AWRAHCQNRD LSQYIRNCGV

Formula weight

14820.48 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS128:SG
2	disulfide	sing	1:CYS30:SG	1:CYS116:SG
3	disulfide	sing	1:CYS65:SG	1:CYS81:SG
4	disulfide	sing	1:CYS77:SG	1:CYS95:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.3 %, Completeness (bb): 99.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	80.3 % (741 of 923)	79.4 % (613 of 772)	84.8 % (128 of 151)
Backbone	99.5 % (393 of 395)	99.6 % (266 of 267)	99.2 % (127 of 128)
Sidechain	65.9 % (348 of 528)	68.7 % (347 of 505)	4.3 % (1 of 23)
Aromatic	83.8 % (62 of 74)	88.6 % (62 of 70)	0.0 % (0 of 4)
Methyl	87.1 % (54 of 62)	87.1 % (54 of 62)

1. mouse lysozyme

KVYERCEFAR TLKRNGMAGY YGVSLADWVC LAQHESNYNT RATNYNRGDQ STDYGIFQIN SRYWCNDGKT PRAVNACGIN CSALLQDDIT AAIQCAKRVV RDPQGIRAWV AWRAHCQNRD LSQYIRNCGV

Show sample condition

Sample

Temperature 308 K, pH 3.8 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	mouse lysozyme	[U-15N]		1.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1IVM, Strand ID: A Detail

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Release date

2000-06-04

Citation

Assignment of 1H and 15N resonances of mouse lysozyme M
Obita, T., Ueda, T., Tanaka, Y., Hashimoto, Y., Imoto, T.
J. Biomol. NMR (2000), 18, 361-362, PubMed 11200531 , DOI: , Abstract

Related entities 1. mouse lysozyme, : 1 : 2 : 243 entities Detail

Interaction partners 1. mouse lysozyme, : 7 interactors Detail

Experiments performed 6 experiments Detail