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Found 1 Document (1.736 seconds)

BMRB: 4752

1J9I

1H,13C,15N chemical shift assignments for the DNA binding domain of gpNu1

Authors

De Beer, T., Catalano, C.

Assembly

gpnu1-E68

Entity

1. gpnu1-E68 (polymer, Thiol state: not present), 68 monomers, 7808.685 × 2 Da Detail

MEVNKKQLAD IFGASIRTIQ NWQEQGMPVL RGGGKGNEVL YDSAAVIKWY AERDAEIENE KLRREVEE

Total weight

15617.37 Da

Max. entity weight

7808.685 Da

Source organism

Escherichia virus Lambda

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.6 % (795 of 806)	98.6 % (419 of 425)	99.0 % (301 of 304)	97.4 % (75 of 77)
Backbone	98.5 % (400 of 406)	97.9 % (138 of 141)	99.0 % (196 of 198)	98.5 % (66 of 67)
Sidechain	98.9 % (457 of 462)	98.9 % (281 of 284)	99.4 % (167 of 168)	90.0 % (9 of 10)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (23 of 23)	100.0 % (2 of 2)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. gpnu1 DNA binding domain

MEVNKKQLAD IFGASIRTIQ NWQEQGMPVL RGGGKGNEVL YDSAAVIKWY AERDAEIENE KLRREVEE

Show sample condition

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1J9I, Strand ID: A, B Detail

Release date

2000-09-26

Citation

Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the DNA binding domain of gpNu1, a genome packaging protein from bacteriophage l
de Beer, T., Ortega, M., Berton, N., Yang, Q., Overduin, M., Catalano, C.E.
J. Biomol. NMR (2000), 18, 69-70, PubMed , DOI:

Related entities 1. gpnu1-E68, : 1 : 4 : 2 : 1 : 4 entities Detail

Interaction partners 1. gpnu1-E68, : 7 interactors Detail

More details for 7 interactors identified by 3 citations

Experiments performed 17 experiments Detail

1 2D 1H NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

2 2D 1H TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

3 1H-15N HSQC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

4 1H-15N HSQC-NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

5 1H-15N HMQC-NOESY-HMQC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

6 1H-15N HSQC-DIPSI

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

7 1H-15N HNHA

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

8 1H-13C HSQCs

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

9 3D 1H-13C HMQC-NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

10 4D 1H-13C HMQC-NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

11 2D (HB)CB(CGCD)HD

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

12 2D HCCH-TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

13 CBCA(CO)NNH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

14 HNCACB

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

15 C-TOCSY-(CO)NNH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

16 H(C-TOCSY)-(CO)NNH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

17 HNCO

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpnu1 DNA binding domain			0.5 ~ 1.0 mM
2	Tris	[U-2H]		20 mM

Sample #2

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	gpnu1 DNA binding domain	[U-98% 15N]		1.0 mM
4	Tris	[U-2H]		20 mM

Sample #3

Pressure 0.2 (±0.02) atm, Temperature 298 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	gpnu1 DNA binding domain	[U-98% 13C; U-98% 15N]		1.0 mM

nullKeywords gpnu1, assignment, DNA, ATP

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Found 1 Document (1.736 seconds)

BMRB: 4752

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. gpnu1-E68, : 1 : 4 : 2 : 1 : 4 entities Detail

Interaction partners 1. gpnu1-E68, : 7 interactors Detail

Experiments performed 17 experiments Detail

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