BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.0 % (449 of 554)	90.3 % (261 of 289)	67.9 % (144 of 212)	83.0 % (44 of 53)
Backbone	82.1 % (230 of 280)	94.7 % (90 of 95)	69.1 % (96 of 139)	95.7 % (44 of 46)
Sidechain	82.4 % (263 of 319)	88.1 % (171 of 194)	78.0 % (92 of 118)	0.0 % (0 of 7)
Aromatic	39.6 % (19 of 48)	79.2 % (19 of 24)	0.0 % (0 of 24)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. Ubiquitin associated domain

GSQEKEAIER LKALGFPESL VIQAYFACEK NENLAANFLL SQNFDDE

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Protein Blocks Logo

Calculated from 18 models in PDB: 1DV0, Strand ID: A Detail

Release date

2000-06-26

Citation

Biochemical and Structural Analysis of the Interaction Between the UBA(2) Domain of the DNA Repair Protein HHR23A and HIV-1 Vpr
Withers-Ward, E.S., Mueller, T.D., Chen, I.S.Y., Feigon, J.
Biochemistry (2000), 39, 14103-14112, PubMed , DOI:

Related entities 1. C-terminal UBA Domain of HHR23A, : 1 : 12 : 69 entities Detail

Interaction partners 1. C-terminal UBA Domain of HHR23A, : 41 interactors Detail

More details for 41 interactors identified by 16 citations

Experiments performed 12 experiments Detail

1 3D 1H-1H-15N NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

2 3D 1H-1H-15N TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

3 3D 15N-1H-15N HSQC-NOE-HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

4 3D CBCA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

5 3D CBCANH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

6 3D HBHA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

7 3D 1H-13C-1H HCCH-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

8 3D 1H-13C-1H HCCH-TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

9 3D 1H-13C-1H NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

10 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

11 2D DQF-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

12 2D TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin associated domain	[U-95% 13C; U-95% 15N]		2.5 mM

Sample #2

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ubiquitin associated domain	[U-95% 15N]		2.5 mM

Sample #3

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin associated domain			2.5 mM

nullKeywords UBA Domain, DNA Repair, HIV-1 Vpr, cell cycle arrest

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Found 1 Document (2.139 seconds)

BMRB: 4757

Sample

Related entities 1. C-terminal UBA Domain of HHR23A, : 1 : 12 : 69 entities Detail

Interaction partners 1. C-terminal UBA Domain of HHR23A, : 41 interactors Detail

Experiments performed 12 experiments Detail

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