BMRBj - BMREntryMaster

Found 1 Document (3.202 seconds)

BMRB: 4775

1DUJ

Solution structure of the spindle assembly checkpoint protein human MAD2

Authors

Luo, X., Fang, G., Coldiron, M., Lin, Y., Yu, H.

Assembly

SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2

Entity

1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2 (polymer, Thiol state: all free), 187 monomers, 21408.16 Da Detail

GSITLRGSAE IVAEFFSFGI NSILYQRGIY PSETFTRVQK YGLTLLVTTD LELIKYLNNV VEQLKDWLYK CSVQKLVVVI SNIESGEVLE RWQFDIECDK TAKDDSAPRE KSQKAIQDEI RSVIRQITAT VTFLPLLEVS CSFDLLIYTD KDLVVPEKWE ESGPQFITNS EEVRLRSFTT TIHKVNS

Formula weight

21408.16 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 80.6 %, Completeness (bb): 94.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.6 % (1809 of 2244)	85.6 % (993 of 1160)	70.2 % (621 of 884)	97.5 % (195 of 200)
Backbone	94.9 % (1055 of 1112)	95.5 % (359 of 376)	93.5 % (518 of 554)	97.8 % (178 of 182)
Sidechain	70.2 % (921 of 1312)	80.9 % (634 of 784)	52.9 % (270 of 510)	94.4 % (17 of 18)
Aromatic	35.4 % (63 of 178)	68.5 % (61 of 89)	0.0 % (0 of 86)	66.7 % (2 of 3)
Methyl	89.2 % (223 of 250)	95.2 % (119 of 125)	83.2 % (104 of 125)

1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.4 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.4 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1DUJ, Strand ID: A Detail

Release date

2000-08-03

Citation

Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20
Luo, X., Fang, G., Coldiron, M., Lin, Y., Yu, H., Kirschner, M.W., Wagner, G.
Nat. Struct. Biol. (2000), 7, 224-229, PubMed 10700282 , DOI 10.1038/73338 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q13257 released on 2001-01-11
Title MD2L1_HUMAN Entity Mitotic spindle assembly checkpoint protein MAD2A

Related entities 1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2, : 1 : 2 : 15 : 29 entities Detail

Interaction partners 1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2, : 51 interactors Detail

More details for 51 interactors identified by 24 citations

Experiments performed 6 experiments Detail

1 3D 15N-NOESY

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

2 3D 13C-NOESY

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

3 3D HCCH TOCSY

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

4 3D 15N-TOCSY

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

5 3D H(CC)(CO)NH

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

6 3D (H)C(C)(CO)NH

Instrument

#1 Model Varian INOVA (750 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-2H]	1.2 mM
2	sodium phosphate		50 mM
3	KCl		300 mM
4	DTT		5 mM
5	NaN3		0.04 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
8	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N; U-13C; U-60% 2H]	1.4 mM
9	sodium phosphate		50 mM
10	KCl		300 mM
11	DTT		5 mM
12	NaN3		0.04 %
13	H2O		90 %
14	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
15	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-15N]	1.5 mM
16	sodium phosphate		50 mM
17	KCl		300 mM
18	DTT		5 mM
19	NaN3		0.04 %
20	H2O		90 %
21	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	[U-10% 13C]	1.6 mM
23	sodium phosphate		50 mM
24	KCl		300 mM
25	DTT		5 mM
26	NaN3		0.04 %
27	H2O		90 %
28	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Concentration
29	SPINDLE ASSEMBLY CHECKPOINT PROTEIN	1.2 mM
30	sodium phosphate	50 mM
31	KCl	300 mM
32	DTT	5 mM
33	NaN3	0.04 %
34	D2O	100 %

nullKeywords Mad2, spindle assembly checkpoint

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Found 1 Document (3.202 seconds)

BMRB: 4775

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2, : 1 : 2 : 15 : 29 entities Detail

Interaction partners 1. SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2, : 51 interactors Detail

Experiments performed 6 experiments Detail

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