BMRB: 4778

Backbone 1HN, 15N and 13C shifts for GMPPNP-loaded Cdc42 from Candida albicans

Authors

Osborne, M.J., Stevens, W.K., Ping, X., Ni, F.

Assembly

Cell Division Control Protein 42 from Candida albicans

Entity

1. Cell division control protein 42 (polymer, Thiol state: all free), 181 monomers, 20106.80 Da Detail

GSHMQTIKCV VVGDGAVGKT CLLISYTTSK FPADYVPTVF DNYAVTVMIG DEPFTLGLFD TAGQEDYDRL RPLSYPSTDV FLVCFSVISP ASFENVKEKW FPEVHHHCPG VPIIIVGTQT DLRNDDVILQ RLHRQKLSPI TQEQGEKLAK ELRAVKYVEC SALTQRGLKT VFDEAIVAAL E

2. 5p-guanosyl-imido-triphosphate (non-polymer), 1 monomers, 522.196 Da

Total weight

20628.996 Da

Max. entity weight

20106.8 Da

Source organism

Candida albicans

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.8 %, Completeness: 36.5 %, Completeness (bb): 58.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	36.5 % (764 of 2094)	25.8 % (278 of 1076)	40.7 % (340 of 835)	79.8 % (146 of 183)
Backbone	58.0 % (618 of 1066)	47.1 % (171 of 363)	56.8 % (302 of 532)	84.8 % (145 of 171)
Sidechain	21.7 % (260 of 1198)	15.0 % (107 of 713)	32.1 % (152 of 473)	8.3 % (1 of 12)
Aromatic	9.4 % (16 of 170)	10.6 % (9 of 85)	8.3 % (7 of 84)	0.0 % (0 of 1)
Methyl	15.0 % (35 of 234)	12.0 % (14 of 117)	17.9 % (21 of 117)

1. Cell division control protein 42

GSHMQTIKCV VVGDGAVGKT CLLISYTTSK FPADYVPTVF DNYAVTVMIG DEPFTLGLFD TAGQEDYDRL RPLSYPSTDV FLVCFSVISP ASFENVKEKW FPEVHHHCPG VPIIIVGTQT DLRNDDVILQ RLHRQKLSPI TQEQGEKLAK ELRAVKYVEC SALTQRGLKT VFDEAIVAAL E

Show sample condition

Sample

Temperature 298 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	Cell division control protein 42	[U-15N; U-13C]		0.7 mM
2	5p-guanosyl-imido-triphosphate			7 mM
3	sodium phosphate			50 mM
4	magnesium chloride			2 mM
5	sodium chloride			50 mM

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LACS Plot; CA

Referencing offset: 0.5 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.5 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Release date

2004-02-12

Citation

Efficient expression of isotopically labeled peptides for high resolution NMR studies: application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans
Osborne, M.J., Su, Z., Sridaran, V., Ni, F.
J. Biomol. NMR (2003), 26, 317-326, PubMed 12815258 , DOI: , Abstract

Related entities 1. Cell division control protein 42, : 2 : 298 entities Detail

Interaction partners 1. Cell division control protein 42, : 1 interactors Detail

Experiments performed 3 experiments Detail