BMRBj - BMREntryMaster

Found 1 Document (3.126 seconds)

BMRB: 4791

1JR6

Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase

Authors

Liu, D., Wyss, D.F.

Assembly

HCV NS3 RNA helicase

Entity

1. HCV NS3 RNA helicase (polymer, Thiol state: not reported), 142 monomers, 15128.09 Da Detail

GSHMGSVTVP HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT NGDVVVVATD ALMTGFTGDF DSVIDCNTSD GKPQDAVSRT QRRGRTGRGK PGIYRFVAPG ER

Formula weight

15128.09 Da

Source organism

Hepacivirus C

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 95.0 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.0 % (1486 of 1565)	96.0 % (774 of 806)	93.2 % (577 of 619)	96.4 % (135 of 140)
Backbone	95.5 % (798 of 836)	96.9 % (284 of 293)	93.9 % (384 of 409)	97.0 % (130 of 134)
Sidechain	94.8 % (810 of 854)	95.5 % (490 of 513)	94.0 % (315 of 335)	83.3 % (5 of 6)
Aromatic	85.7 % (84 of 98)	91.8 % (45 of 49)	79.6 % (39 of 49)
Methyl	98.8 % (168 of 170)	98.8 % (84 of 85)	98.8 % (84 of 85)

1. HCV helicase

GSHMGSVTVP HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT NGDVVVVATD ALMTGFTGDF DSVIDCNTSD GKPQDAVSRT QRRGRTGRGK PGIYRFVAPG ER

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	na	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	0.0
¹⁵N	DSS	na	external	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	na	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	0.0
¹⁵N	DSS	na	external	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	na	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	0.0
¹⁵N	DSS	na	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	na	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	0.0
¹⁵N	DSS	na	external	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1JR6, Strand ID: A Detail

Release date

2001-05-02

Citation

Letter to the editor: Sequence-specific 1H, 15N and 13C resonance assignments for an engineered arginine-rich domain of the hepatitis C virus NS3 RNA helicase
Liu, D., Wyss, D.F.
J. Biomol. NMR (2000), 18, 279-280, PubMed 11142518 , DOI: , Abstract

Related entities 1. HCV NS3 RNA helicase, : 1 : 1 : 97 entities Detail

Interaction partners 1. HCV NS3 RNA helicase, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

2 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

3 HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

4 HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

5 (H)CC(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

6 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

7 15N-edited TOCSY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
2	KPO4		75 mM
3	DTT	[U-2H]	5 mM
4	D2O		99 %
5	AEBSF		0.4 mM
6	NaN3		0.015 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	HCV helicase	[U-15N; U-13C]	0.2 ~ 1.0 mM
8	KPO4		75 mM
9	DTT	[U-2H]	5 mM
10	D2O		5 %
11	AEBSF		0.4 mM
12	NaN3		0.015 %

nullKeywords HCV, helicase, NMR assignments

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Found 1 Document (3.126 seconds)

BMRB: 4791

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HCV NS3 RNA helicase, : 1 : 1 : 97 entities Detail

Interaction partners 1. HCV NS3 RNA helicase, : 1 interactors Detail

Experiments performed 7 experiments Detail

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