BMRBj - BMREntryMaster

Found 1 Document (2.925 seconds)

BMRB: 4792

2AN7

Backbone NMR Assignment and Secondary Structure of the Dimeric ParD Protein

Authors

Oberer, M., Prytulla, S., Keller, W.

Assembly

ParD dimer

Entity

1. ParD dimer (polymer, Thiol state: not present), 83 monomers, 9103.160 × 2 Da Detail

MSRLTIDMTD QQHQSLKALA ALQGKTIKQY ALERLFPGDA DADQAWQELK TMLGNRINDG LAGKVSTKSV GEILDEELSG DRA

Total weight

18206.32 Da

Max. entity weight

9103.16 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 85.5 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.5 % (804 of 940)	80.0 % (392 of 490)	89.9 % (322 of 358)	97.8 % (90 of 92)
Backbone	96.4 % (478 of 496)	94.8 % (163 of 172)	97.1 % (235 of 242)	97.6 % (80 of 82)
Sidechain	76.9 % (400 of 520)	72.0 % (229 of 318)	83.9 % (161 of 192)	100.0 % (10 of 10)
Aromatic	50.0 % (17 of 34)	82.4 % (14 of 17)	12.5 % (2 of 16)	100.0 % (1 of 1)
Methyl	84.4 % (81 of 96)	79.2 % (38 of 48)	89.6 % (43 of 48)

1. ParD

MSRLTIDMTD QQHQSLKALA ALQGKTIKQY ALERLFPGDA DADQAWQELK TMLGNRINDG LAGKVSTKSV GEILDEELSG DRA

Show sample condition

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.57 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.57 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 24 models in PDB: 2AN7, Strand ID: A, B Detail

Release date

2002-04-03

Citation 1

The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins
Oberer, M., Zangger, K., Prytulla, S., Keller, W.
Biochem. J. (2002), 361, 41-47, PubMed 11743881 , DOI 10.1042/bj3610041 , Abstract

Show more 1 citaion

Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 1 citaion

Related entities 1. ParD dimer, : 1 : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H15N HSQC

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

2 2D NOESY

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

3 2D TOCSY

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

4 3D 15N NOESY-HSQC

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

5 3D 15N TOCSY-HSQC

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

6 3D HNCO

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

7 3D HN(CO)CA

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

8 3D CBCA(CO)NH

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

9 3D HNCACB

Instrument

Model Varian Unity INOVA (600 MHz)

Sample #1

Temperature 306 K, pH 6.0, Details Degradation of the sample was observed after prolonged measurement

#	Name	Isotope labeling	Type	Concentration
1	ParD	[U-98% 13C; U-98% 15N]		0.6 mM

Sample #2

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
2	ParD	[U-98% 15N]		0.5 mM

Sample #3

Temperature 306 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	ParD			0.5 mM

nullKeywords plasmid addiction system, antitoxin, RK2/RP4, autoregulation, NMR

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Found 1 Document (2.925 seconds)

BMRB: 4792

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ParD dimer, : 1 : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

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