BMRB: 4797

Sequence-specific resonance assignments of the potent cytolysin equinatoxin II

Authors

Zhang, W., Hinds, M.G., Anderluh, G., Hansen, P.E., Norton, R.S.

Assembly

EqtII monomer

Entity

1. EqtII monomer (polymer, Thiol state: not present), 179 monomers, 19833.22 Da Detail

SADVAGAVID GASLSFDILK TVLEALGNVK RKIAVGVDNE SGKTWTALNT YFRSGTSDIV LPHKVPHGKA LLYNGQKDRG PVATGAVGVL AYLMSDGNTL AVLFSVPYDY NWYSNWWNVR IYKGKRRADQ RMYEELYYNL SPFRGDNGWH TRNLGYGLKS RGFMNSSGHA ILEIHVTKA

Formula weight

19833.22 Da

Source organism

Actinia equina

Exptl. method

NMR

Refine. method

torsion angle dyanamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 90.5 %, Completeness (bb): 94.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.5 % (1867 of 2062)	93.2 % (988 of 1060)	86.5 % (700 of 809)	92.7 % (179 of 193)
Backbone	94.5 % (1006 of 1064)	97.0 % (361 of 372)	92.5 % (479 of 518)	95.4 % (166 of 174)
Sidechain	87.5 % (1013 of 1158)	91.1 % (627 of 688)	82.7 % (373 of 451)	68.4 % (13 of 19)
Aromatic	62.4 % (136 of 218)	79.8 % (87 of 109)	44.2 % (46 of 104)	60.0 % (3 of 5)
Methyl	94.6 % (193 of 204)	96.1 % (98 of 102)	93.1 % (95 of 102)

1. Equinatoxin II

SADVAGAVID GASLSFDILK TVLEALGNVK RKIAVGVDNE SGKTWTALNT YFRSGTSDIV LPHKVPHGKA LLYNGQKDRG PVATGAVGVL AYLMSDGNTL AVLFSVPYDY NWYSNWWNVR IYKGKRRADQ RMYEELYYNL SPFRGDNGWH TRNLGYGLKS RGFMNSSGHA ILEIHVTKA

Show sample condition

Sample #1

Temperature 303 (±0.2) K, pH 3.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Equinatoxin II	[U-95% 13C; U-95% 15N]		1.0 mM

Sample #2

Temperature 303 (±0.2) K, pH 3.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Equinatoxin II	[U-95% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 0.68 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.68 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 1.35 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1KD6, Strand ID: A Detail

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Release date

2000-11-28

Citation

Letter to the editor: Sequence-specific resonance assignments of the potent cytolysin equinatoxin II
Zhang, W., Hinds, M.G., Anderluh, G., Hansen, P.E., Norton, R.S.
J. Biomol. NMR (2000), 18, 281-282, PubMed 11142519 , DOI: , Abstract

Related entities 1. EqtII monomer, : 1 : 3 : 39 entities Detail