BMRB: 4798

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Frq1

Authors

Ames, J.B., Hendricks, K.B., Strahl, T., Huttner, I.G., Hamasaki, N., Thorner, J.

Assembly

yeast frequenin

Entity

1. Yeast frequenin (polymer, Thiol state: all free), 190 monomers, 22010.72 Da Detail

MGAKTSKLSK DDLTCLKQST YFDRREIQQW HKGFLRDCPS GQLAREDFVK IYKQFFPFGS PEDFANHLFT VFDKDNNGFI HFEEFITVLS TTSRGTLEEK LSWAFELYDL NHDGYITFDE MLTIVASVYK MMGSMVTLNE DEATPEMRVK KIFKLMDKNE DGYITLDEFR EGSKVDPSII GALNLYDGLI

2. CA (non-polymer), 40.078 × 3 Da

Total weight

22130.955 Da

Max. entity weight

22010.72 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 75.3 %, Completeness: 45.5 %, Completeness (bb): 69.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	45.5 % (1043 of 2291)	42.1 % (501 of 1189)	46.0 % (415 of 903)	63.8 % (127 of 199)
Backbone	69.3 % (783 of 1130)	70.8 % (274 of 387)	68.6 % (383 of 558)	68.1 % (126 of 185)
Sidechain	28.8 % (386 of 1339)	28.3 % (227 of 802)	30.2 % (158 of 523)	7.1 % (1 of 14)
Aromatic	5.9 % (15 of 256)	6.3 % (8 of 128)	5.6 % (7 of 126)	0.0 % (0 of 2)
Methyl	20.7 % (39 of 188)	21.3 % (20 of 94)	20.2 % (19 of 94)

1. Yeast frequenin

MGAKTSKLSK DDLTCLKQST YFDRREIQQW HKGFLRDCPS GQLAREDFVK IYKQFFPFGS PEDFANHLFT VFDKDNNGFI HFEEFITVLS TTSRGTLEEK LSWAFELYDL NHDGYITFDE MLTIVASVYK MMGSMVTLNE DEATPEMRVK KIFKLMDKNE DGYITLDEFR EGSKVDPSII GALNLYDGLI

Show sample condition

Sample

Temperature 318 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Yeast frequenin	[U-95% 13C; U-90% 15N]		0.8 mM
2	octyl glucoside			20 mM

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.44 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1FPW, Strand ID: A Detail

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Release date

2000-09-02

Citation

Structure and calcium-binding properties of Frq1, a novel calcium sensor in the yeast Saccharomyces cerevisiae
Ames, J.B., Hendricks, K.B., Strahl, T., Huttner, I.G., Hamasaki, N., Thorner, J.
Biochemistry (2000), 39, 12149-12161, PubMed 11015193 , DOI: , Abstract

Related entities 1. Yeast frequenin, : 1 : 3 : 336 entities Detail

Interaction partners 1. Yeast frequenin, : 38 interactors Detail

Experiments performed 5 experiments Detail