BMRB: 4814

Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III

Authors

Fan, D., Reese, L., Ren, X., Weers, P.M., Ryan, R.O., Wang, J.

Assembly

Locust apolipophorin-III

Entity

1. Locust apolipophorin-III (polymer, Thiol state: not present), 164 monomers, 17580.08 Da Detail

RPDAAGHVNI AEAVQQLNHT IVNAAHELHE TLGLPTPDEA LNLLTEQANA FKTKIAEVTT SLKQEAEKHQ GSVAEQLNAF ARNLNNSIHD AATSLNLQDQ LNSLQSALTN VGHQWQDIAT KTQASAQEAW APVQSALQEA AEKTKEAAAN LQNSIQSAVQ KPAN

Formula weight

17580.08 Da

Source organism

Locusta migratoria

Exptl. method

NMR

Refine. method

Simulated Annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 78.4 %, Completeness (bb): 84.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.4 % (1426 of 1818)	95.0 % (889 of 936)	54.9 % (378 of 688)	82.0 % (159 of 194)
Backbone	84.5 % (823 of 974)	97.2 % (318 of 327)	71.3 % (348 of 488)	98.7 % (157 of 159)
Sidechain	75.7 % (760 of 1004)	93.6 % (570 of 609)	52.2 % (188 of 360)	5.7 % (2 of 35)
Aromatic	38.9 % (28 of 72)	72.2 % (26 of 36)	5.9 % (2 of 34)	0.0 % (0 of 2)
Methyl	71.2 % (148 of 208)	99.0 % (103 of 104)	43.3 % (45 of 104)

1. apolipoprotein

RPDAAGHVNI AEAVQQLNHT IVNAAHELHE TLGLPTPDEA LNLLTEQANA FKTKIAEVTT SLKQEAEKHQ GSVAEQLNAF ARNLNNSIHD AATSLNLQDQ LNSLQSALTN VGHQWQDIAT KTQASAQEAW APVQSALQEA AEKTKEAAAN LQNSIQSAVQ KPAN

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	apolipoprotein	[U-99% 13C; U-99% 15N]		0.6 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 21 models in PDB: 1LS4, Strand ID: A Detail

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Release date

2001-02-13

Citation 1

Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III
Fan, D., Reese, L., Ren, X., Weers, P.M.M., Ryan, R.O., Wang, J.
J. Biomol. NMR (2001), 19, 83-84, PubMed 11246860 , DOI: , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

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Related entities 1. Locust apolipophorin-III, : 1 : 1 : 3 entities Detail

Experiments performed 2 experiments Detail