BMRB: 4816

Structural Features of an Influenza Virus Promoter and their Implications for Viral RNA Synthesis

Authors

Bae, S., Cheong, H., Lee, J., Cheong, C., Kainosho, M., Choi, B.

Assembly

INFLUENZA A VIRUS PROMOTER RNA

Entity

1. INFLUENZA A VIRUS PROMOTER RNA (polymer, Thiol state: not present), 31 monomers, 9975.865 Da Detail

AGUAGAAACA AGGCUUCGGC CUGCUUUUGC U

Formula weight

9975.865 Da

Source organism

Influenza A virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 29.6 %, Completeness (bb): 32.8 % Detail

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Polymer type: polyribonucleotide

	Total	13C	15N	31P
All	29.6 % (76 of 257)	18.7 % (39 of 209)	88.2 % (15 of 17)	71.0 % (22 of 31)
Suger, PO4	32.8 % (61 of 186)	25.2 % (39 of 155)		71.0 % (22 of 31)
Nucleobase	21.1 % (15 of 71)	0.0 % (0 of 54)	88.2 % (15 of 17)
Aromatic	21.1 % (15 of 71)	0.0 % (0 of 54)	88.2 % (15 of 17)

1. INFLUENZA A VIRUS PROMOTER RNA

AGUAGAAACA AGGCUUCGGC CUGCUUUUGC U

Show sample condition

Sample #1

Temperature 278 (±2) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	INFLUENZA A VIRUS PROMOTER RNA			2 mM
2	Na phosphate			10 mM
3	EDTA			0.1 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Temperature 278 (±2) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	INFLUENZA A VIRUS PROMOTER RNA			2 mM
7	Na phosphate			10 mM
8	EDTA			0.1 mM
9	D2O			99.96 %

Sample #3

Temperature 278 (±2) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
10	INFLUENZA A VIRUS PROMOTER RNA	[U-13C; U-15N]		0.4 mM
11	Na phosphate			10 mM
12	EDTA			0.1 mM
13	H2O			90 %
14	D2O			10 %

Sample #4

Temperature 278 (±2) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
15	INFLUENZA A VIRUS PROMOTER RNA	[U-13C; U-15N]		0.4 mM
16	Na phosphate			10 mM
17	EDTA			0.1 mM
18	D2O			99.96 %

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Release date

2002-04-03

Citation

Structural features of an influenza virus promoter and their implications for viral RNA synthesis
Bae, S., Cheong, H., Lee, J., Cheong, C., Kainosho, M., Choi, B.
Proc. Natl. Acad. Sci. U. S. A. (2001), 98, 10602-10607, PubMed 11553808 , DOI 10.1073/pnas.191268798 , Abstract

Related entities 1. INFLUENZA A VIRUS PROMOTER RNA, : 1 : 1 : 1 : 5 entities Detail

Experiments performed 3 experiments Detail