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BMRB: 4825

1M58

1H, 15N and 13C NMR Resonance Assignments of RC-RNase 2

Authors

Hsu, C., Chen, L., Liao, Y., Wu, S., Chen, C.

Assembly

Recombinant RC-RNase 2

Entity

1. Recombinant RC-RNase 2 (polymer, Thiol state: all disulfide bound), 106 monomers, 12346.29 Da Detail

MQNWETFQKK HLTDTRDVKC DAEMKKALFD CKQKNTFIYA RPGRVQALCK NIIVSKNVLS TDEFYLSDCN RIKLPCHYKL KKSSNTICIT CENKLPVHFV AVEECP

Formula weight

12346.29 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS20:SG	1:CYS69:SG
2	disulfide	sing	1:CYS31:SG	1:CYS76:SG
3	disulfide	sing	1:CYS49:SG	1:CYS91:SG
4	disulfide	sing	1:CYS88:SG	1:CYS105:SG

Source organism

Lithobates catesbeianus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.5 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.5 % (1262 of 1294)	97.2 % (661 of 680)	98.4 % (492 of 500)	95.6 % (109 of 114)
Backbone	98.9 % (621 of 628)	98.6 % (206 of 209)	99.4 % (315 of 317)	98.0 % (100 of 102)
Sidechain	96.6 % (745 of 771)	96.6 % (455 of 471)	97.6 % (281 of 288)	75.0 % (9 of 12)
Aromatic	95.9 % (94 of 98)	95.9 % (47 of 49)	95.8 % (46 of 48)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. rRC-RNase2[M(-1),Q1]

MQNWETFQKK HLTDTRDVKC DAEMKKALFD CKQKNTFIYA RPGRVQALCK NIIVSKNVLS TDEFYLSDCN RIKLPCHYKL KKSSNTICIT CENKLPVHFV AVEECP

Show sample condition

Sample

Temperature 310 (±0.5) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	rRC-RNase2[M(-1),Q1]	[U-15N; U-13C]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.58 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1M58, Strand ID: A Detail

Release date

2000-11-12

Citation

1H, 15N and 13C resonance assignments and secondary structure determination of the RC-RNase 2 from oocytes of bullfrog Rana catesbeiana
Hsu, C., Chen, L., Liao, Y., Wu, S., Chen, C.
J. Biomol. NMR (2001), 19, 87-88, PubMed 11246862 , DOI: , Abstract

Related entities 1. Recombinant RC-RNase 2, : 1 : 106 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-13C HSQC