BMRB: 4847

NMR solution structure of alpha-conotoxin Im1 point mutation variant D5N

Authors

Rogers, J.P., Luginbuhl, P., Pemberton, K., Harty, P., Wemmer, D.E., Stevens, R.C.

Assembly

alpha-conotoxin Im1(D5N)

Entity

1. alpha-conotoxin Im1(D5N) (polymer, Thiol state: all disulfide bound), 13 monomers, 1353.600 Da Detail

GCCSNPRCAW RCX

Formula weight

1353.6 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS8:SG
2	disulfide	sing	1:CYS3:SG	1:CYS12:SG

Source organism

Conus imperialis

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMISATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 98.5 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.5 % (66 of 67)	98.5 % (66 of 67)
Backbone	95.8 % (23 of 24)	95.8 % (23 of 24)
Sidechain	100.0 % (43 of 43)	100.0 % (43 of 43)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. alpha-conotoxin Im1(D5N)

GCCSNPRCAW RCX

Show sample condition

Sample

Temperature 298 (±1) K, pH 4.1 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	alpha-conotoxin Im1(D5N)			2.0 ~ 3.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1E76, Strand ID: A Detail

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Release date

2008-07-15

Citation

Structure-activity Relationships in a Peptidic Alpha7 Nicotinic Acetylcholine Receptor Antagonist
Rogers, J.P., Luginbuhl, P., Pemberton, K., Harty, P., Wemmer, D.E., Stevens, R.C.
J. Mol. Biol. (2000), 304, 911-926, PubMed , DOI:

Related entities 1. alpha-conotoxin Im1(D5N), : 1 : 22 entities Detail

Interaction partners 1. alpha-conotoxin Im1(D5N), : 1 interactors Detail

Experiments performed 3 experiments Detail