BMRB: 4850

Glutaredoxin 3 from Escherichia coli in the fully oxidized form

Authors

Nordstrand, K., Sandstrom, A., Aslund, F., Holmgren, A., Otting, G., Berndt, K.D.

Assembly

GLUTAREDOXIN 3

Entity

1. GLUTAREDOXIN 3 (polymer, Thiol state: all disulfide bound), 82 monomers, 9066.231 Da Detail

ANVEIYTKET CPYCHRAKAL LSSKGVSFQE LPIDGNAAKR EEMIKRSGRT TVPQIFIDAQ HIGGYDDLYA LDARGGLDPL LK

Formula weight

9066.231 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS11:SG	1:CYS14:SG

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing in torsion angle space followed by conjugate gradient minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	97.7 % (560 of 573)	98.4 % (482 of 490)	94.0 % (78 of 83)
Backbone	96.3 % (236 of 245)	97.6 % (163 of 167)	93.6 % (73 of 78)
Sidechain	98.8 % (324 of 328)	98.8 % (319 of 323)	100.0 % (5 of 5)
Aromatic	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	100.0 % (46 of 46)	100.0 % (46 of 46)

1. GLUTAREDOXIN 3

ANVEIYTKET CPYCHRAKAL LSSKGVSFQE LPIDGNAAKR EEMIKRSGRT TVPQIFIDAQ HIGGYDDLYA LDARGGLDPL LK

Show sample condition

Sample #1

Temperature 301 (±0.2) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	GLUTAREDOXIN 3			0.0 ~ 0.0 mM

Sample #2

Temperature 301 (±0.2) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	GLUTAREDOXIN 3	[U-15N]		0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1FOV, Strand ID: A Detail

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Release date

2001-03-11

Citation

NMR structure of oxidized glutaredoxin 3 from Escherichia coli
Nordstrand, K., Sandstrom, A., Aslund, F., Holmgren, A., Otting, G., Berndt, K.D.
J. Mol. Biol. (2000), 303, 423-432, PubMed 11031118 , DOI 10.1006/jmbi.2000.4145 , Abstract

Related entities 1. GLUTAREDOXIN 3, : 1 : 3 : 257 entities Detail