BMRB: 4869

HMG PROTEIN NHP6A FROM SACCHAROMYCES CEREVISIAE

Authors

Allain, F.H.T., Yen, Y.M., Masse, J.E., Schultze, P., Dieckmann, T., Johnson, R.C., Feigon, J.

Assembly

NON HISTONE PROTEIN 6 A

Entity

1. NON HISTONE PROTEIN 6 A (polymer, Thiol state: not present), 93 monomers, 10802.18 Da Detail

MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ VGKKLGEKWK ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA

Formula weight

10802.18 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.1 %, Completeness: 73.3 %, Completeness (bb): 75.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.3 % (839 of 1144)	78.8 % (484 of 614)	62.3 % (271 of 435)	88.4 % (84 of 95)
Backbone	75.4 % (410 of 544)	87.9 % (160 of 182)	63.0 % (174 of 276)	88.4 % (76 of 86)
Sidechain	73.5 % (507 of 690)	75.0 % (324 of 432)	70.3 % (175 of 249)	88.9 % (8 of 9)
Aromatic	86.5 % (64 of 74)	100.0 % (37 of 37)	72.2 % (26 of 36)	100.0 % (1 of 1)
Methyl	91.7 % (66 of 72)	94.4 % (34 of 36)	88.9 % (32 of 36)

1. PROTEIN (NON HISTONE PROTEIN 6 A)

MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ VGKKLGEKWK ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA

Show sample condition

Sample

Temperature 293 (±1.0) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN (NON HISTONE PROTEIN 6 A)	[U-13C; U-15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 2.99 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 2.99 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1LWM, Strand ID: A Detail

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Release date

2001-11-13

Citation

Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-sequence-specific binding
Allain, F.H.T., Yen, Y.M., Masse, J.M., Schultze, P., Dieckmann, T., Johnson, R.C., Feigon, J.
EMBO J. (1999), 18, 2563-2579, PubMed 10228169 , DOI 10.1093/emboj/18.9.2563 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. NON HISTONE PROTEIN 6 A, : 1 : 4 : 209 entities Detail

Interaction partners 1. NON HISTONE PROTEIN 6 A, : 16 interactors Detail

Experiments performed 6 experiments Detail