BMRBj - BMREntryMaster

Found 1 Document (4.172 seconds)

BMRB: 4884

1G47

1st LIM domain of PINCH protein

Authors

Velyvis, A., Yang, Y., Wu, C., Qin, J.

Assembly

1st LIM domain of PINCH protein

Entity

1. 1st LIM domain of PINCH protein (polymer, Thiol state: all other bound), 77 monomers, 8845.012 Da Detail

ISEFMANALA SATCERCKGG FAPAEKIVNS NGELYHEQCF VCAQCFQQFP EGLFYEFEGR KYCEHDFQML FAPCWIL

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8975.83 Da

Max. entity weight

8845.012 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	2:ZN1:ZN	1:CYS14:SG
2	metal coordination	sing	2:ZN1:ZN	1:CYS17:SG
3	metal coordination	sing	2:ZN1:ZN	1:HIS36:ND1
4	metal coordination	sing	2:ZN1:ZN	1:CYS39:SG
5	metal coordination	sing	2:ZN1:ZN	1:CYS42:SG
6	metal coordination	sing	2:ZN1:ZN	1:CYS45:SG
7	metal coordination	sing	2:ZN1:ZN	1:CYS63:SG
8	metal coordination	sing	2:ZN1:ZN	1:HIS65:ND1

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.7 %, Completeness: 71.5 %, Completeness (bb): 72.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.5 % (655 of 916)	78.7 % (374 of 475)	59.5 % (213 of 358)	81.9 % (68 of 83)
Backbone	72.8 % (332 of 456)	85.3 % (133 of 156)	60.2 % (136 of 226)	85.1 % (63 of 74)
Sidechain	72.2 % (384 of 532)	75.5 % (241 of 319)	67.6 % (138 of 204)	55.6 % (5 of 9)
Aromatic	47.8 % (64 of 134)	55.2 % (37 of 67)	40.9 % (27 of 66)	0.0 % (0 of 1)
Methyl	69.0 % (40 of 58)	69.0 % (20 of 29)	69.0 % (20 of 29)

1. 1st LIM domain of PINCH protein

ISEFMANALA SATCERCKGG FAPAEKIVNS NGELYHEQCF VCAQCFQQFP EGLFYEFEGR KYCEHDFQML FAPCWIL

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1G47, Strand ID: A Detail

Release date

2001-03-11

Citation

Solution structure of the focal adhesion adaptor PINCH LIM1 domain and characterization of its interaction with the integrin-linked kinase ankyrin repeat domain
Velyvis, A., Yang, Y., Wu, C., Qin, J.
J. Biol. Chem. (2001), 276, 4932-4939, PubMed 11078733 , DOI 10.1074/jbc.M007632200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P48059 released on 1996-02-01
Title LIMS1_HUMAN Entity LIM and senescent cell antigen-like-containing domain protein 1

Related entities 1. 1st LIM domain of PINCH protein, : 1 : 9 : 132 entities Detail

Interaction partners 1. 1st LIM domain of PINCH protein, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

2 3D CBCANH

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

3 3D CBCA(CO)NH

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

4 3D 1H-1H-13C/15N NOESY

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

5 3D 1H-13C-1H HCCH-TOCSY

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

6 3D C(CO)NH

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

7 3D H(CCO)NH

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

8 3D HNHA

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

9 2D 1H-1H NOESY

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

10 2D 1H-1H TOCSY

Instrument

Model Varian Unity Plus (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Concentration
1	1st LIM domain of PINCH protein	0.5 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	beta-mercaptoethanol	0.5 mM

Sample #2

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	1st LIM domain of PINCH protein	[U-15N]	0.5 mM
6	Na phosphate		50 mM
7	NaCl		100 mM
8	beta-mercaptoethanol		0.5 mM

Sample #3

Temperature 298 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	1st LIM domain of PINCH protein	[U-15N; U-13C]	0.5 mM
10	Na phosphate		50 mM
11	NaCl		100 mM
12	beta-mercaptoethanol		0.5 mM

null

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (4.172 seconds)

BMRB: 4884

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. 1st LIM domain of PINCH protein, : 1 : 9 : 132 entities Detail

Interaction partners 1. 1st LIM domain of PINCH protein, : 2 interactors Detail

Experiments performed 10 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3