BMRB: 4890

1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit

Authors

Gervais, V., Wasielewski, E., Busso, D., Poterszman, A., Egly, J., Thierry, J., Kieffer, B.

Assembly

CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1

Entity

1. Menage a trois (polymer, Thiol state: not reported), 65 monomers, 7296.417 Da Detail

MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFED

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7427.235 Da

Max. entity weight

7296.417 Da

Entity Connection

metal coordination 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	2:ZN1:ZN	1:CYS6:SG
2	metal coordination	sing	2:ZN1:ZN	1:CYS9:SG
3	metal coordination	sing	2:ZN1:ZN	1:CYS31:SG
4	metal coordination	sing	2:ZN1:ZN	1:CYS34:SG
5	metal coordination	sing	2:ZN1:ZN	1:CYS26:SG
6	metal coordination	sing	2:ZN1:ZN	1:HIS28:ND1
7	metal coordination	sing	2:ZN1:ZN	1:CYS46:SG
8	metal coordination	sing	2:ZN1:ZN	1:CYS49:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 92.6 %, Completeness (bb): 94.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.6 % (361 of 390)	92.6 % (361 of 390)
Backbone	94.7 % (124 of 131)	94.7 % (124 of 131)
Sidechain	91.5 % (237 of 259)	91.5 % (237 of 259)
Aromatic	85.7 % (18 of 21)	85.7 % (18 of 21)
Methyl	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Menage a trois

MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFED

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Sample

Temperature 298 (±0.1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Menage a trois			2 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1G25, Strand ID: A Detail

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Release date

2000-12-03

Citation 1

Solution structure of the N-terminal domain of the human TFIIH MAT1 subunit: new insights into the RING finger family
Gervais, V., Busso, D., Wasielewski, E., Poterszman, A., Egly, J., Thierry, J., Kieffer, B.
J. Biol. Chem. (2001), 276, 7457-7464, PubMed 11056162 , DOI 10.1074/jbc.M007963200 , Abstract

Show more 3 citaions

Citation 2

Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor
Wu, L., Yee, A., Nichols, M.A., Hall, F.L., Kobayashi, R., Xiong, Y.
Cancer. Res. (1995), 55, 6058-6062, PubMed 8521393 , Abstract

Citation 3

X-PLOR, version 3.1: A system for X-ray crystallography and NMR
Brunger, A.T.

Citation 4

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
Bartels, C., Xia, T., Billeter, M., Guntert, P., Wuthrich, K.
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 3 citaions

Related entities 1. Menage a trois, : 1 : 22 : 93 entities Detail

Interaction partners 1. Menage a trois, : 3 interactors Detail

Experiments performed 3 experiments Detail