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BMRB: 4893

1KVZ

1H, 15N and 13C NMR Resonance Assignments of RC-RNase 4

Authors

Hsu, C., Liao, Y., Wu, S., Chen, C.

Assembly

Recombinant RC-RNase 4

Entity

1. Recombinant RC-RNase 4 (polymer, Thiol state: all disulfide bound), 107 monomers, 12217.14 Da Detail

MQDWATFKKK HLTDTWDVDC DNLMPTSLFD CKDKNTFIYS LPGPVKALCR GVIFSADVLS NSEFYLAECN VKPRKPCKYK LKKSSNRICI RCEHELPVHF AGVGICP

Formula weight

12217.14 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS20:SG	1:CYS69:SG
2	disulfide	sing	1:CYS31:SG	1:CYS77:SG
3	disulfide	sing	1:CYS49:SG	1:CYS92:SG
4	disulfide	sing	1:CYS89:SG	1:CYS106:SG

Source organism

Lithobates catesbeianus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.1 % (1256 of 1280)	97.9 % (655 of 669)	98.4 % (495 of 503)	98.1 % (106 of 108)
Backbone	98.4 % (618 of 628)	98.1 % (207 of 211)	98.7 % (313 of 317)	98.0 % (98 of 100)
Sidechain	96.4 % (728 of 755)	96.5 % (442 of 458)	96.2 % (278 of 289)	100.0 % (8 of 8)
Aromatic	93.3 % (112 of 120)	93.3 % (56 of 60)	93.1 % (54 of 58)	100.0 % (2 of 2)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. rRC-RNase4[M(-1),Q1]

MQDWATFKKK HLTDTWDVDC DNLMPTSLFD CKDKNTFIYS LPGPVKALCR GVIFSADVLS NSEFYLAECN VKPRKPCKYK LKKSSNRICI RCEHELPVHF AGVGICP

Show sample condition

Sample

Temperature 310 (±0.5) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	rRC-RNase4[M(-1),Q1]	[U-15N; U-13C]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1KVZ, Strand ID: A Detail

Release date

2000-11-05

Citation

1H, 13C and 15N resonance assignments and secondary structure of the cytotoxic protein RNase 4 from bullfrog Rana catesbeiana oocytes
Hsu, C., Liao, Y., Wu, S., Chen, C.
J. Biomol. NMR (2001), 20, 93-94, PubMed 11430762 , DOI: , Abstract

Related entities 1. Recombinant RC-RNase 4, : 1 : 88 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-13C HSQC