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Found 1 Document (2.433 seconds)

BMRB: 4894

1EKZ

RNA recognition by a staufen double-stranded RNA-binding domain

Authors

Ramos, A., Grunert, S., Bycroft, M., St Johnston, D., Varani, G.

Assembly

MATERNAL EFFECT PROTEIN (STAUFEN)/RNA COMPLEX

Entity

1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN (polymer, Thiol state: all free), 90 monomers, 9929.493 Da Detail

GSMDEGDKKS PISQVHEIGI KRNMTVHFKV LREEGPAHMK NFITACIVGS IVTEGEGNGK KVSKKRAAEK MLVELQKLPP LTPTKEFIVD

2. RNA hairpin (polymer, Thiol state: not present), 30 monomers, 9675.707 Da Detail

GGACAGCUGU CCCUUCGGGG ACAGCUGUCC

Total weight

19605.2 Da

Max. entity weight

9929.493 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 65.8 %, Completeness: 42.9 %, Completeness (bb): 39.3 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N	³¹P
All	42.9 % (674 of 1572)	51.3 % (423 of 825)	28.6 % (175 of 611)	71.7 % (76 of 106)	0.0 % (0 of 30)
Backbone	39.3 % (350 of 890)	43.8 % (159 of 363)	28.6 % (118 of 412)	85.9 % (73 of 85)	0.0 % (0 of 30)
Sidechain	47.6 % (364 of 764)	57.4 % (265 of 462)	34.2 % (96 of 281)	14.3 % (3 of 21)
Aromatic	13.2 % (23 of 174)	19.5 % (17 of 87)	8.5 % (6 of 71)	0.0 % (0 of 16)
Methyl	85.7 % (84 of 98)	98.0 % (48 of 49)	73.5 % (36 of 49)

1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN

GSMDEGDKKS PISQVHEIGI KRNMTVHFKV LREEGPAHMK NFITACIVGS IVTEGEGNGK KVSKKRAAEK MLVELQKLPP LTPTKEFIVD

2. RNA hairpin

GGACAGCUGU CCCUUCGGGG ACAGCUGUCC

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN			0.0 ~ 0.0 mM
2	RNA hairpin			0.0 ~ 0.0 mM

Chem. Shift Complete2

Sequence coverage: 36.7 %, Completeness: 39.8 %, Completeness (bb): 42.2 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N	³¹P
All	39.8 % (1252 of 3144)	44.4 % (732 of 1650)	34.3 % (419 of 1222)	45.8 % (97 of 212)	6.7 % (4 of 60)
Backbone	42.2 % (752 of 1780)	50.0 % (363 of 726)	36.8 % (303 of 824)	48.2 % (82 of 170)	6.7 % (4 of 60)
Sidechain	36.2 % (553 of 1528)	40.0 % (370 of 924)	29.9 % (168 of 562)	35.7 % (15 of 42)
Aromatic	42.8 % (149 of 348)	46.6 % (81 of 174)	39.4 % (56 of 142)	37.5 % (12 of 32)
Methyl	48.0 % (94 of 196)	54.1 % (53 of 98)	41.8 % (41 of 98)

1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN

GSMDEGDKKS PISQVHEIGI KRNMTVHFKV LREEGPAHMK NFITACIVGS IVTEGEGNGK KVSKKRAAEK MLVELQKLPP LTPTKEFIVD

2. RNA hairpin

GGACAGCUGU CCCUUCGGGG ACAGCUGUCC

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN			0.0 ~ 0.0 mM
2	RNA hairpin			0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UUCG RNA tetraloop	aromatic	105.1 ppm	internal	direct
¹H	UUCG RNA tetraloop	methylene protons	2.78 ppm	internal	direct
¹⁵N	UUCG RNA tetraloop	aromatic	144.8 ppm	internal	direct
³¹P	UUCG RNA tetraloop	phosphorous	-3.6 ppm	internal	direct

Referencing offset: 1.61 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UUCG RNA tetraloop	aromatic	105.1 ppm	internal	direct
¹H	UUCG RNA tetraloop	methylene protons	2.78 ppm	internal	direct
¹⁵N	UUCG RNA tetraloop	aromatic	144.8 ppm	internal	direct
³¹P	UUCG RNA tetraloop	phosphorous	-3.6 ppm	internal	direct

Referencing offset: 1.61 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UUCG RNA tetraloop	aromatic	105.1 ppm	internal	direct
¹H	UUCG RNA tetraloop	methylene protons	2.78 ppm	internal	direct
¹⁵N	UUCG RNA tetraloop	aromatic	144.8 ppm	internal	direct
³¹P	UUCG RNA tetraloop	phosphorous	-3.6 ppm	internal	direct

Referencing offset: -0.26 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 36 models in PDB: 1EKZ, Strand ID: A Detail

Release date

2000-12-05

Citation

RNA recognition by a Staufen double-stranded RNA-binding domain
Ramos, A., Grunert, S., Adams, J., Micklem, D.R., Proctor, M.R., Freund, S., Bycroft, M., St Johnston, D., Varani, G.
EMBO J. (2000), 19, 997-1009, PubMed 10698941 , DOI 10.1093/emboj/19.5.997 , Abstract

Related entities 1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN, : 1 : 98 entities Detail

Related entities 2. RNA hairpin, : 2 entities Detail

Interaction partners 1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN, : 8 interactors Detail

More details for 8 interactors identified by 4 citations

nullKeywords Drosophila, NMR structure, protein dsRBD, protein/RNA, RNA hairpin

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Found 1 Document (2.433 seconds)

BMRB: 4894

Sample

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN, : 1 : 98 entities Detail

Related entities 2. RNA hairpin, : 2 entities Detail

Interaction partners 1. STAUFEN DOUBLE-STRANDED RNA BINDING DOMAIN, : 8 interactors Detail