BMRB: 4895

Solution NMR Structure of the Cold-shock Protein from the Hyperthermophilic Bacterium Thermotoga maritima

Authors

Kremer, W., Schuler, B., Harrieder, S., Geyer, M., Gronwald, W., Welker, C., Jaenicke, R., Kalbitzer, H.

Assembly

TmCsp

Entity

1. TmCsp (polymer, Thiol state: not present), 66 monomers, 7472.462 Da Detail

MRGKVKWFDS KKGYGFITKD EGGDVFVHWS AIEMEGFKTL KEGQVVEFEI QEGKKGPQAA HVKVVE

Formula weight

7472.462 Da

Source organism

Thermotoga maritima

Exptl. method

NMR

Refine. method

dynamic simulated annealing protocol

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 96.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.2 % (722 of 800)	96.4 % (404 of 419)	80.4 % (250 of 311)	97.1 % (68 of 70)
Backbone	96.7 % (381 of 394)	97.9 % (137 of 140)	95.8 % (181 of 189)	96.9 % (63 of 65)
Sidechain	85.7 % (397 of 463)	95.7 % (267 of 279)	69.8 % (125 of 179)	100.0 % (5 of 5)
Aromatic	47.8 % (43 of 90)	91.1 % (41 of 45)	0.0 % (0 of 43)	100.0 % (2 of 2)
Methyl	94.8 % (55 of 58)	89.7 % (26 of 29)	100.0 % (29 of 29)

1. Csp

MRGKVKWFDS KKGYGFITKD EGGDVFVHWS AIEMEGFKTL KEGQVVEFEI QEGKKGPQAA HVKVVE

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Sample

Temperature 303 (±2) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Csp			1.5 mM

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LACS Plot; CA

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; CB

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; CO

Referencing offset: 0.71 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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Protein Blocks Logo

Calculated from 7 models in PDB: 1G6P, Strand ID: A Detail

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Release date

2001-11-13

Citation

Solution NMR structure of the cold-shock protein from the hyperthermophilic bacterium Thermotoga maritima
Kremer, W., Schuler, B., Harrieder, S., Geyer, M., Gronwald, W., Welker, C., Jaenicke, R., Kalbitzer, H.
Eur. J. Biochem. (2001), 268, 2527-2539, PubMed 11322871 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. TmCsp, : 1 : 1 : 214 entities Detail

Experiments performed 6 experiments Detail