BMRB: 4900

1H and 15N chemical shift assignments of the C-terminal xylan binding module of C. fimi xylanase 11A

Authors

Bolam, D.N., Xie, H., White, P., Simpson, P.J., Hancock, S.M., Williamson, M.P., Gilbert, H.J.

Assembly

C-terminal xylan binding module

Entity

1. C-terminal xylan binding module (polymer, Thiol state: all disulfide bound), 88 monomers, 8746.300 Da Detail

TGSCSVSAVR GEEWADRFNV TYSVSGSSSW VVTLGLNGGQ SVQSSWNAAL TGSSGTVTAR PNGSGNSFGV TFYKNGSSAT PGATCATG

Formula weight

8746.3 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS85:SG

Source organism

Cellulomonas fimi

Exptl. method

NMR

Refine. method

HYBRID DISTANCE GEOMETRY/ SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.3 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	99.3 % (533 of 537)	99.8 % (439 of 440)	96.9 % (94 of 97)
Backbone	98.5 % (270 of 274)	99.5 % (187 of 188)	96.5 % (83 of 86)
Sidechain	100.0 % (263 of 263)	100.0 % (252 of 252)	100.0 % (11 of 11)
Aromatic	100.0 % (44 of 44)	100.0 % (41 of 41)	100.0 % (3 of 3)
Methyl	100.0 % (42 of 42)	100.0 % (42 of 42)

1. xylanase 11A

TGSCSVSAVR GEEWADRFNV TYSVSGSSSW VVTLGLNGGQ SVQSSWNAAL TGSSGTVTAR PNGSGNSFGV TFYKNGSSAT PGATCATG

Show sample condition

Sample #1

Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	xylanase 11A			1.0 mM

Sample #2

Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	xylanase 11A	[U-99% 15N]		1.0 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1HEH, Strand ID: C Detail

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Release date

2000-11-20

Citation

Evidence for synergy between family 2b carbohydrate binding modules in Cellulomonas fimi xylanase 11A
Bolam, D.N., Xie, H., White, P., Simpson, P.J., Hancock, S.M., Williamson, M.P., Gilbert, H.J.
Biochemistry (2001), 40, 2468-2477, PubMed 11327868 , DOI: , Abstract

Related entities 1. C-terminal xylan binding module, : 1 : 2 : 13 entities Detail

Experiments performed 5 experiments Detail