BMRB: 4902

Complete sequence-specific 1H, 13C and 15N resonance assignments of a novel hPTK6 SH2 domain

Authors

Hong, E., Shin, J., Lee, W.

Assembly

SH2 domain of Human Protein Tyrosine Kinase 6

Entity

1. SH2 domain of Human Protein Tyrosine Kinase 6 (polymer, Thiol state: all free), 100 monomers, 11343.77 Da Detail

SEPWFFGCIS RSEAVRRLQA EGNATGAFLI RVSEKPSADY VLSVRDTQAV RHYKIWRRAG GRLHLNEAVS FLSLPELVNY HRAQSLSHGL RLAAPCRKHE

Formula weight

11343.77 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.9 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.9 % (1038 of 1167)	87.5 % (532 of 608)	89.7 % (408 of 455)	94.2 % (98 of 104)
Backbone	98.3 % (582 of 592)	95.0 % (192 of 202)	100.0 % (294 of 294)	100.0 % (96 of 96)
Sidechain	82.4 % (551 of 669)	84.0 % (341 of 406)	81.6 % (208 of 255)	25.0 % (2 of 8)
Aromatic	25.9 % (28 of 108)	40.7 % (22 of 54)	9.6 % (5 of 52)	50.0 % (1 of 2)
Methyl	98.2 % (108 of 110)	96.4 % (53 of 55)	100.0 % (55 of 55)

1. SH2 domian of Human Protein Tyrosine Kinase6

SEPWFFGCIS RSEAVRRLQA EGNATGAFLI RVSEKPSADY VLSVRDTQAV RHYKIWRRAG GRLHLNEAVS FLSLPELVNY HRAQSLSHGL RLAAPCRKHE

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.005)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domian of Human Protein Tyrosine Kinase6	[U-98% 13C; U-99.8% 15N]		0.5 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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Protein Blocks Logo

Calculated from 21 models in PDB: 1RJA, Strand ID: A Detail

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Release date

2001-04-25

Citation

Complete sequence-specific 1H, 13C and 15N resonance assignments of the human PTK6 SH2 domain
Hong, E., Shin, J., Bang, E., Kim, M., Lee, S., Lee, W.
J. Biomol. NMR (2001), 19, 291-292, PubMed 11330822 , Abstract

Related entities 1. SH2 domain of Human Protein Tyrosine Kinase 6, : 1 : 2 : 300 entities Detail

Interaction partners 1. SH2 domain of Human Protein Tyrosine Kinase 6, : 21 interactors Detail

Experiments performed 15 experiments Detail