BMRB: 4907

NMR Solution Structure of Phospholamban

Authors

Lamberth, S., Schmid, H., Muenchbach, M., Vorherr, T., Krebs, J., Carafoli, E., Griesinger, C.

Assembly

Phospholamban (C41F)

Entity

1. Phospholamban (C41F) (polymer, Thiol state: all free), 52 monomers, 6124.462 Da Detail

MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI FLLLICIIVM LL

Formula weight

6124.462 Da

Source organism

Sus scrofa

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.8 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	83.8 % (290 of 346)	83.8 % (290 of 346)
Backbone	100.0 % (103 of 103)	100.0 % (103 of 103)
Sidechain	77.0 % (187 of 243)	77.0 % (187 of 243)
Aromatic	26.3 % (5 of 19)	26.3 % (5 of 19)
Methyl	77.8 % (35 of 45)	77.8 % (35 of 45)

1. Phospholamban

MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI FLLLICIIVM LL

Show sample condition

Sample

Temperature 300 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Phospholamban			1.0 mM
2	CDCl3	[U-2H]		50 % v/v
3	CD3OH			50 % v/v

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Calculated from 10 models in PDB: 1FJP, Strand ID: A Detail

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Release date

2000-12-21

Citation

NMR Solution Structure of Phospholamban
Griesinger, C.
Helv. Chim. Acta (2000), 83, 2141-2152, DOI:

Related entities 1. Phospholamban (C41F), : 1 : 1 : 3 : 14 entities Detail

Interaction partners 1. Phospholamban (C41F), : 1 interactors Detail

Experiments performed 3 experiments Detail