BMRBj - BMREntryMaster

Found 1 Document (1.59 seconds)

BMRB: 4910

1HDL

1H and 15N chemical shift assignments for LEKTI domain one (HF6478)

Authors

Lauber, T., Adermann, K., Schulz, A., Forssmann, W.GEORG., Roesch, P., Marx, U.CHARLOTTE.

Assembly

LEKTI domain one

Entity

1. LEKTI domain one (polymer, Thiol state: all disulfide bound), 55 monomers, 6481.536 Da Detail

KNEDQEMCHE FQAFMKNGKL FCPQDKKFFQ SLDGIMFINK CATCKMILEK EAKSQ

Formula weight

6481.536 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS44:SG
2	disulfide	sing	1:CYS22:SG	1:CYS41:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 89.1 %, Completeness: 80.8 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	80.8 % (349 of 432)	80.0 % (296 of 370)	85.5 % (53 of 62)
Backbone	87.9 % (145 of 165)	87.4 % (97 of 111)	88.9 % (48 of 54)
Sidechain	76.4 % (204 of 267)	76.8 % (199 of 259)	62.5 % (5 of 8)
Aromatic	50.0 % (16 of 32)	50.0 % (16 of 32)
Methyl	62.5 % (10 of 16)	62.5 % (10 of 16)

1. HF6478

KNEDQEMCHE FQAFMKNGKL FCPQDKKFFQ SLDGIMFINK CATCKMILEK EAKSQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

Chem. Shift Complete2

Sequence coverage: 94.5 %, Completeness: 80.4 %, Completeness (bb): 77.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	80.4 % (695 of 864)	85.5 % (633 of 740)	50.0 % (62 of 124)
Backbone	77.0 % (254 of 330)	89.2 % (198 of 222)	51.9 % (56 of 108)
Sidechain	82.6 % (441 of 534)	84.0 % (435 of 518)	37.5 % (6 of 16)
Aromatic	65.6 % (42 of 64)	65.6 % (42 of 64)
Methyl	81.3 % (26 of 32)	81.3 % (26 of 32)

1. HF6478

KNEDQEMCHE FQAFMKNGKL FCPQDKKFFQ SLDGIMFINK CATCKMILEK EAKSQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

Protein Blocks Logo

Calculated from 21 models in PDB: 1HDL, Strand ID: A Detail

Coupling constant

13 J values in 1 lists
Temperature 298 (±1) K, pH 4.5 (±0.2) Detail

Release date

2000-12-05

Citation 1

Homologous proteins with different folds: the three-dimensional structures of domains 1 and 6 of the multiple Kazal-type inhibitor LEKTI
Lauber, T., Schulz, A., Schweimer, K., Adermann, K., Marx, U.CHARLOTTE.
J. Mol. Biol. (2003), 328, 205-219, PubMed 12684009 , DOI: , Abstract

Show more 1 citaion

Citation 2

NMRView: A computer program for the visualization and analysis of NMR data
Johnson, B.A., Blevins, R.A.
J. Biomol. NMR (1994), 4, 603-614

Hide non-primary 1 citaion

Related entities 1. LEKTI domain one, : 1 : 2 : 1 : 83 entities Detail

Experiments performed 8 experiments Detail

1 2D-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

2 2D-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

3 2D-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

4 1H-15N-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

5 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

6 3D-1H-15N-TOCSY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

7 3D-1H-15N-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

8 3D-1H-15N/1H-15N-HMQC-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HF6478	[U-15N]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	HF6478			1.2 mM

null

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.59 seconds)

BMRB: 4910

Sample #1

Sample #2

Sample #1

Sample #2

Related entities 1. LEKTI domain one, : 1 : 2 : 1 : 83 entities Detail

Experiments performed 8 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2