BMRBj - BMREntryMaster

Found 1 Document (1.862 seconds)

BMRB: 4920

2M66

Thioredoxin fold as a Homodimerization Module in the Putative Chaperone ERp29: NMR Structures of the Domains and Experimental Model of the 51 kDa Dimer

Authors

Liepinsh, E., Baryshev, M., Sharipo, A., Ingelman-Sundberg, M., Otting, G., Mkrtchian, S.

Assembly

ERp29 C-domain

Entity

1. ERp29 C-domain (polymer, Thiol state: all free), 120 monomers, 13481.26 Da Detail

MRGSHHHHHH GIRMPGCLPA YDALAGQFIE ASSREARQAI LKQGQDGLSG VKETDKKWAS QYLKIMGKIL DQGEDFPASE LARISKLIEN KMSEGKKEEL QRSLNILTAF RKKGAEKEEL

Formula weight

13481.26 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

PCS-Rosetta simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 77.7 %, Completeness (bb): 83.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.7 % (1100 of 1416)	94.9 % (712 of 750)	49.5 % (267 of 539)	95.3 % (121 of 127)
Backbone	83.8 % (598 of 714)	95.2 % (236 of 248)	71.9 % (251 of 349)	94.9 % (111 of 117)
Sidechain	64.9 % (526 of 811)	94.8 % (476 of 502)	13.4 % (40 of 299)	100.0 % (10 of 10)
Aromatic	37.8 % (31 of 82)	73.2 % (30 of 41)	0.0 % (0 of 40)	100.0 % (1 of 1)
Methyl	56.4 % (62 of 110)	98.2 % (54 of 55)	14.5 % (8 of 55)

1. Endoplasmic reticulum protein p29

MRGSHHHHHH GIRMPGCLPA YDALAGQFIE ASSREARQAI LKQGQDGLSG VKETDKKWAS QYLKIMGKIL DQGEDFPASE LARISKLIEN KMSEGKKEEL QRSLNILTAF RKKGAEKEEL

Show sample condition

Sample

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Protein Blocks Logo

Calculated from 11 models in PDB: 2M66, Strand ID: A Detail

Release date

2001-08-07

Citation

Thioredoxin fold as homodimerization module in the putative chaperone ERp29: NMR structures of the domains and experimental model of the 51 kDa dimer
Liepinsh, E., Baryshev, M., Sharipo, A., Ingelman-Sundberg, M., Otting, G., Mkrtchian, S.
Structure (2001), 9, 457-471, PubMed 11435111 , DOI: , Abstract

Related entities 1. ERp29 C-domain, : 1 : 4 : 19 entities Detail

Interaction partners 1. ERp29 C-domain, : 5 interactors Detail

More details for 5 interactors identified by 2 citations

Experiments performed 8 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

2 2D 1H-1H COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

3 2D 1H-1H CLEAN TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

4 2D 1H-15N HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

5 3D 1H-1H-15N NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

6 3D HNCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

7 3D HN(CO)CA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

8 3D HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Endoplasmic reticulum protein p29	[U-15N; U-13C]		2 mM

Sample #2

Temperature 308 (±1) K, pH 4.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Endoplasmic reticulum protein p29			2 mM

nullKeywords Nuclear magnetic resonance spectroscopy, protein structure

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Found 1 Document (1.862 seconds)

BMRB: 4920

Sample

Related entities 1. ERp29 C-domain, : 1 : 4 : 19 entities Detail

Interaction partners 1. ERp29 C-domain, : 5 interactors Detail

Experiments performed 8 experiments Detail

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