BMRB: 4924

Interactions of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (SqKv1A).

Authors

Ellis, K.C., Tenenholz, T.C., Gilly, W., Blaustein, M.P., Weber, D.J.

Assembly

Tityustoxin K-alpha

Entity

1. Tityustoxin K-alpha (polymer, Thiol state: all disulfide bound), 37 monomers, 3947.779 Da Detail

VFINAKCRGS PECLPKCKEA IGKAAGKCMN GKCKCYP

Formula weight

3947.779 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS28:SG
2	disulfide	sing	1:CYS13:SG	1:CYS33:SG
3	disulfide	sing	1:CYS17:SG	1:CYS35:SG

Source organism

Tityus serrulatus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 89.4 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	89.4 % (202 of 226)	89.4 % (202 of 226)
Backbone	96.0 % (72 of 75)	96.0 % (72 of 75)
Sidechain	86.1 % (130 of 151)	86.1 % (130 of 151)
Aromatic	88.9 % (8 of 9)	88.9 % (8 of 9)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. Tityustoxin K-alpha

VFINAKCRGS PECLPKCKEA IGKAAGKCMN GKCKCYP

Show sample condition

Sample

Pressure 1 atm, Temperature 310.00 (±0.05) K, pH 3.50 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Tityustoxin K-alpha			4.53 mM

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Protein Blocks Logo

Calculated from 30 models in PDB: 1HP2, Strand ID: A Detail

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Release date

2000-12-12

Citation

Interactions of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (SqKv1A)
Ellis, K.C., Tenenholz, T.C., Gilly, W., Blaustein, M.P., Weber, D.J.
Biochemistry (2001), 40, 5942-5953, PubMed , DOI:

Related entities 1. Tityustoxin K-alpha, : 1 : 2 : 1 : 163 entities Detail

Experiments performed 5 experiments Detail