BMRB: 4925

Structural Basis for the Functional switch of the E. Coli Ada Protein

Authors

Lin, Y., Dotsch, V., Wintner, T., Peariso, K., Myers, L.C., Penner-Hahn, J.E., Verdine, G.L., Wagner, G.

Assembly

N-Ada10

Entity

1. N-Ada10 (polymer, Thiol state: not reported), 92 monomers, 10449.81 Da Detail

MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPDKANPR QHRLDKITHA CR

2. ZN (non-polymer), 65.409 Da

Total weight

10515.219 Da

Max. entity weight

10449.81 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS38:SG	2:ZN1:ZN
2	na	sing	1:CYS42:SG	2:ZN1:ZN
3	na	sing	1:CYS69:SG	2:ZN1:ZN
4	na	sing	1:CYS72:SG	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 65.4 %, Completeness (bb): 65.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	65.4 % (691 of 1057)	87.6 % (487 of 556)	28.1 % (114 of 405)	93.8 % (90 of 96)
Backbone	65.7 % (356 of 542)	94.0 % (171 of 182)	37.7 % (103 of 273)	94.3 % (82 of 87)
Sidechain	56.3 % (340 of 604)	82.9 % (310 of 374)	9.5 % (21 of 221)	100.0 % (9 of 9)
Aromatic	51.2 % (42 of 82)	95.1 % (39 of 41)	5.0 % (2 of 40)	100.0 % (1 of 1)
Methyl	52.5 % (42 of 80)	92.5 % (37 of 40)	12.5 % (5 of 40)

1. N-Ada10

MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPDKANPR QHRLDKITHA CR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	N-Ada10	[U-15N; U-13C]		2.0 ~ 3.0 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1EYF, Strand ID: A Detail

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Release date

2001-09-11

Citation

Structural basis for the functional switch of the E. coli Ada protein
Lin, Y., Dotsch, V., Wintner, T., Peariso, K., Myers, L.C., Penner-Hahn, J.E., Verdine, G.L., Wagner, G.
Biochemistry (2001), 40, 4261-4271, PubMed 11284682 , DOI: , Abstract

Related entities 1. N-Ada10, : 1 : 1 : 2 : 6 entities Detail

Interaction partners 1. N-Ada10, : 8 interactors Detail

Experiments performed 4 experiments Detail