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Found 1 Document (1.549 seconds)

BMRB: 4927

1N02

1H, 13C, 15N Resonance Assignments of a Circular Permuted Variant of CV-N (cpCV-N)

Authors

Barrientos, L.G., Campos-Olivas, R., Gronenborn, A.M.

Assembly

Circular Permuted Variant of CV-N

Entity

1. Circular Permuted Variant of CV-N (polymer, Thiol state: all disulfide bound), 102 monomers, 11070.06 Da Detail

GLGKFIETCR NTQLAGSSEL AAECKTRAQQ FVSTKINLDD HIANIDGTLK WQPSNFSQTC YNSAIQGSVL TSTCERTNGG YNTSSIDLNS VIENVDGSLK YE

Formula weight

11070.06 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS24:SG
2	disulfide	sing	1:CYS60:SG	1:CYS74:SG

Source organism

Nostoc ellipsosporum

Exptl. method

NMR

Refine. method

for dyana, Standard target function-simulated annealing protocol. For CNS, Simulated-annealing in Cartesian space

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.4 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.4 % (1053 of 1127)	91.7 % (532 of 580)	94.9 % (408 of 430)	96.6 % (113 of 117)
Backbone	96.9 % (591 of 610)	97.2 % (205 of 211)	97.0 % (289 of 298)	96.0 % (97 of 101)
Sidechain	90.7 % (554 of 611)	88.6 % (327 of 369)	93.4 % (211 of 226)	100.0 % (16 of 16)
Aromatic	65.7 % (46 of 70)	65.7 % (23 of 35)	64.7 % (22 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. Cyanovirin-N

GLGKFIETCR NTQLAGSSEL AAECKTRAQQ FVSTKINLDD HIANIDGTLK WQPSNFSQTC YNSAIQGSVL TSTCERTNGG YNTSSIDLNS VIENVDGSLK YE

Show sample condition

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	proton	4.754 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	proton	4.754 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	proton	4.754 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	proton	4.754 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 26 models in PDB: 1N02, Strand ID: A Detail

Release date

2002-04-03

Citation

1H, 13C, 15N resonance assignments and fold verification of a circular permuted variant of the potent HIV-inactivating protein cyanovirin-N
Barrientos, L.G., Campos-Olivas, R., Louis, J.M., Fiser, A., Sali, A., Gronenborn, A.M.
J. Biomol. NMR (2001), 19, 289-290, PubMed 11330821 , DOI: , Abstract

Related entities 1. Circular Permuted Variant of CV-N, : 1 : 39 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

2 2D 1H-13C HSQC/HMQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

3 3D HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

4 3D HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

5 3D CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

6 3D HCCH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

7 3D DIPSI-H(CCO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

8 3D HNHA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

9 3D 15N-edited NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

10 4D 13C,13C NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cyanovirin-N	[U-15N]		0.3 ~ 1.0 mM
2	phosphate buffer			20 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Cyanovirin-N	[U-15N; U-13C]		0.3 ~ 1.0 mM
4	phosphate buffer			20 mM

nullKeywords Circular permutation, cyanovirin-N, heteronuclear NMR, HIV-1, residual dipolar couplings, resonance assignments

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Found 1 Document (1.549 seconds)

BMRB: 4927

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Chemical shift reference

Related entities 1. Circular Permuted Variant of CV-N, : 1 : 39 entities Detail

Experiments performed 10 experiments Detail

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