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BMRB: 4929

1XDX

The 1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain from Chlamydomonas flagella

Authors

Wu, H., Maciejewski, M.W., Benashski, S.E., Mullen, G.P., King, S.M.

Assembly

Tctex1 dimer

Entity

1. Tctex1 dimer (polymer, Thiol state: all free), 114 monomers, 12668.32 × 2 Da Detail

MEGVDPAVEE AAFVADDVSN IIKESIDAVL QNQQYSEAKV SQWTSSCLEH CIKRLTALNK PFKYVVTCII MQKNGAGLHT AASCWWDSTT DGSRTVRWEN KSMYCICTVF GLAI

Total weight

25336.64 Da

Max. entity weight

12668.32 Da

Source organism

Chlamydomonas reinhardtii

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.7 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.7 % (903 of 1296)	56.9 % (377 of 663)	81.7 % (414 of 507)	88.9 % (112 of 126)
Backbone	98.4 % (669 of 680)	97.4 % (225 of 231)	99.1 % (334 of 337)	98.2 % (110 of 112)
Sidechain	46.9 % (340 of 725)	35.2 % (152 of 432)	66.7 % (186 of 279)	14.3 % (2 of 14)
Aromatic	0.0 % (0 of 110)	0.0 % (0 of 55)	0.0 % (0 of 51)	0.0 % (0 of 4)
Methyl	61.9 % (83 of 134)	59.7 % (40 of 67)	64.2 % (43 of 67)

1. Chlamydomonas Tctex1

MEGVDPAVEE AAFVADDVSN IIKESIDAVL QNQQYSEAKV SQWTSSCLEH CIKRLTALNK PFKYVVTCII MQKNGAGLHT AASCWWDSTT DGSRTVRWEN KSMYCICTVF GLAI

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.7 (±0.2), Details the dimer concentration is 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	Chlamydomonas Tctex1	[U-97% 13C; U-99% 15N]		1.4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1XDX, Strand ID: A, B Detail

Release date

2001-05-16

Citation

1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain from Chlamydomonas flagella
Wu, H., Maciejewski, M.W., Benashski, S.E., Mullen, G.P., King, S.M.
J. Biomol. NMR (2001), 20, 89-90, PubMed 11430760 , DOI: , Abstract

Related entities 1. Tctex1 dimer, : 1 : 1 : 96 entities Detail

Experiments performed 13 experiments Detail

1 HNCA (6388 x 4400 x 1418 Hz, 512 x 55 x 30, 48 scans)