Backbone Assignments for Af19
MKESVVWTVN LDSKKSRAEG RRIPRRFAVP NVKLHELVEA SKELGLKFRA EEKKYPKSWW EEGGRVVVEK RGTKTKLMIE LARKIAEIRE QKREQKKDKK KKKK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.2 % (1126 of 1337) | 83.3 % (602 of 723) | 85.4 % (432 of 506) | 85.2 % (92 of 108) |
Backbone | 90.9 % (562 of 618) | 90.0 % (189 of 210) | 91.9 % (282 of 307) | 90.1 % (91 of 101) |
Sidechain | 80.1 % (655 of 818) | 80.5 % (413 of 513) | 80.9 % (241 of 298) | 14.3 % (1 of 7) |
Aromatic | 10.3 % (7 of 68) | 17.6 % (6 of 34) | 0.0 % (0 of 31) | 33.3 % (1 of 3) |
Methyl | 100.0 % (98 of 98) | 100.0 % (49 of 49) | 100.0 % (49 of 49) |
1. Archaeglobus fulgidus SRP19
MKESVVWTVN LDSKKSRAEG RRIPRRFAVP NVKLHELVEA SKELGLKFRA EEKKYPKSWW EEGGRVVVEK RGTKTKLMIE LARKIAEIRE QKREQKKDKK KKKKTemperature 300 (±1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Archaeglobus fulgidus SRP19 | [U-99% 13C; U-98% 15N] | 1.8 ~ 2.2 mM | |
2 | NaCl | 0.050 M | ||
3 | KH2PO4 | 0.025 M | ||
4 | D2O | 99.88 % |
Temperature 300 (±1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Archaeglobus fulgidus SRP19 | [U-98% 15N] | 1.8 ~ 2.2 mM | |
6 | NaCl | 0.050 M | ||
7 | KH2PO4 | 0.025 M | ||
8 | D2O | 5 % | ||
9 | H2O | 95 % |