BMRB: 4938

Two Different Neurodegenerative Diseases Caused by Proteins with Similiar Structures

Authors

Mo, H., Moore, R.C., Cohen, F.E., Westway, D., Prusiner, S.B., Wright, P.E., Dyson, H.JANE.

Assembly

mouse doppel

Entity

1. mouse doppel (polymer, Thiol state: all disulfide bound), 132 monomers, 15099.88 Da Detail

ARGIKHRFKW NRKVLPSSGG QITEARVAEN RPGAFIKQGR KLDIDFGAEG NRYYAANYWQ FPDGIYYEGC SEANVTKEML VTSCVNATQA ANQAEFSREK QDSKLHQRVL WRLIKEICSA KHCDFWLERG AA

Formula weight

15099.88 Da

Entity Connection

disulfide 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS70:SG	1:CYS123:SG
2	disulfide	sing	0:CYS84:SG	1:CYS118:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry molecular dynamics simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 97.6 %, Completeness (bb): 97.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.6 % (1527 of 1564)	97.4 % (799 of 820)	97.7 % (583 of 597)	98.6 % (145 of 147)
Backbone	97.3 % (765 of 786)	97.0 % (263 of 271)	97.2 % (375 of 386)	98.4 % (127 of 129)
Sidechain	98.0 % (882 of 900)	97.6 % (536 of 549)	98.5 % (328 of 333)	100.0 % (18 of 18)
Aromatic	98.1 % (157 of 160)	97.5 % (78 of 80)	98.7 % (75 of 76)	100.0 % (4 of 4)
Methyl	98.3 % (116 of 118)	96.6 % (57 of 59)	100.0 % (59 of 59)

1. mouse doppel

ARGIKHRFKW NRKVLPSSGG QITEARVAEN RPGAFIKQGR KLDIDFGAEG NRYYAANYWQ FPDGIYYEGC SEANVTKEML VTSCVNATQA ANQAEFSREK QDSKLHQRVL WRLIKEICSA KHCDFWLERG AA

Show sample condition

Sample

Temperature 299 (±1) K, pH 5.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	mouse doppel	[U-15N]		3 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1I17, Strand ID: A Detail

---

Release date

2001-08-21

Citation

Two different neurodegenerative diseases caused by proteins with similar structures
Mo, H., Moore, R.C., Cohen, F.E., Westaway, D., Prusiner, S.B., Wright, P.E., Dyson, H.JANE.
Proc. Natl. Acad. Sci. U. S. A. (2001), 98, 2352-2357, PubMed 11226243 , DOI 10.1073/pnas.051627998 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. mouse doppel, : 1 : 2 : 6 entities Detail

Interaction partners 1. mouse doppel, : 1 interactors Detail