BMRB: 4939

Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions

Authors

Kowalski, K., Mackay, J.P.

Assembly

ninth zinc-finger domain of the u-shaped transcription factor

Entity

1. U-shaped transcriptional cofactor (polymer, Thiol state: all other bound), 36 monomers, 4167.804 Da Detail

GSAAEVMKKY CSTCDISFNY VKTYLAHKQF YCKNKP

2. ZN (non-polymer), 65.409 Da

Total weight

4233.2134 Da

Max. entity weight

4167.804 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS11:SG	2:ZN1:ZN
2	na	sing	1:CYS14:SG	2:ZN1:ZN
3	na	sing	1:CYS32:SG	2:ZN1:ZN
4	na	sing	1:HIS27:NE2	2:ZN1:ZN

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

molecular dynamics simulated annealing torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 83.1 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	83.1 % (221 of 266)	82.0 % (187 of 228)	89.5 % (34 of 38)
Backbone	94.4 % (101 of 107)	93.1 % (67 of 72)	97.1 % (34 of 35)
Sidechain	75.5 % (120 of 159)	76.9 % (120 of 156)	0.0 % (0 of 3)
Aromatic	57.1 % (16 of 28)	57.1 % (16 of 28)
Methyl	92.3 % (12 of 13)	92.3 % (12 of 13)

1. U-shaped transcriptional cofactor

GSAAEVMKKY CSTCDISFNY VKTYLAHKQF YCKNKP

Show sample condition

Sample

Pressure 1 atm, Temperature 293 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U-shaped transcriptional cofactor			0.4 mM
2	ZnSO4			0.6 mM
3	TCEP			0.6 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1FU9, Strand ID: A Detail

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Release date

2001-01-22

Citation

Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions
Liew, C.K., Kowalski, K., Fox, A.H., Newton, A., Sharpe, B.K., Crossley, M., Mackay, J.P.
Structure (2000), 8, 1157-1166, PubMed 11080638 , DOI 10.1016/s0969-2126(00)00527-x , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. U-shaped transcriptional cofactor, : 1 : 1 : 31 entities Detail

Experiments performed 3 experiments Detail