BMRB: 4945

VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE

Authors

Dulubova, I., Yamaguchi, T., Wang, Y., Sudhof, T.C., Rizo, J.

Assembly

Vam3p N-terminal domain

Entity

1. Vam3p N-terminal domain (polymer, Thiol state: all free), 123 monomers, 14155.83 Da Detail

GSSQQEPQFS TNQKTKELSN LIETFAEQSR VLEKECTKIG SKRDSKELRY KIETELIPNC TSVRDKIESN ILIHQNGKLS ADFKNLKTKY QSLQQSYNQR KSLFPLKTPI SPGTSKERKD IHP

Formula weight

14155.83 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 92.5 %, Completeness (bb): 97.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (1392 of 1505)	94.5 % (760 of 804)	89.2 % (506 of 567)	94.0 % (126 of 134)
Backbone	97.5 % (708 of 726)	96.7 % (236 of 244)	98.1 % (358 of 365)	97.4 % (114 of 117)
Sidechain	89.3 % (802 of 898)	93.6 % (524 of 560)	82.9 % (266 of 321)	70.6 % (12 of 17)
Aromatic	50.0 % (36 of 72)	91.7 % (33 of 36)	8.3 % (3 of 36)
Methyl	99.1 % (109 of 110)	98.2 % (54 of 55)	100.0 % (55 of 55)

1. single chain biopolimer

GSSQQEPQFS TNQKTKELSN LIETFAEQSR VLEKECTKIG SKRDSKELRY KIETELIPNC TSVRDKIESN ILIHQNGKLS ADFKNLKTKY QSLQQSYNQR KSLFPLKTPI SPGTSKERKD IHP

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolimer	[U-99% 15N]		0.9 mM
2	phosphate buffer			20 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	single chain biopolimer	[U-99% 13C; U-99% 15N]		0.85 mM
4	phosphate buffer			20 mM

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LACS Plot; CA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1HS7, Strand ID: A Detail

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Release date

2001-08-21

Citation

Vam3p structure reveals conserved and divergent properties of syntaxins
Dulubova, I., Yamaguchi, T., Wang, Y., Sudhof, T.C., Rizo, J.
Nat. Struct. Biol. (2001), 8, 258-264, PubMed 11224573 , DOI 10.1038/85012 , Abstract

Related entities 1. Vam3p N-terminal domain, : 2 entities Detail

Interaction partners 1. Vam3p N-terminal domain, : 13 interactors Detail

Experiments performed 4 experiments Detail