BMRB: 4950

Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein

Authors

Geyer, M., Kalbitzer, H.R.

Assembly

HIV-1 Nef

Entity

1. HIV-1 Nef (polymer, Thiol state: all free), 57 monomers, 5808.383 Da Detail

GGKWSKSSVV GWPAVRERMR RAEPAADGVG AASRDLEKHG AITSSNTAAN NAACAWX

Formula weight

5808.383 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 99.0 %, Completeness (bb): 97.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	99.0 % (297 of 300)	99.0 % (297 of 300)
Backbone	97.4 % (113 of 116)	97.4 % (113 of 116)
Sidechain	100.0 % (184 of 184)	100.0 % (184 of 184)
Aromatic	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (26 of 26)	100.0 % (26 of 26)

1. HIV-1 Negative Factor

GGKWSKSSVV GWPAVRERMR RAEPAADGVG AASRDLEKHG AITSSNTAAN NAACAWX

Show sample condition

Sample

Temperature 285 (±1) K, pH 4.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 Negative Factor			1.0 mM

Hide sample codition

Protein Blocks Logo

Calculated from 2 models in PDB: 1QA4, Strand ID: A Detail

---

Release date

2008-07-16

Citation 1

Structure of the anchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein
Geyer, M., Munte, C.E., Schorr, J., Kellner, R., Kalbitzer, H.R.
J. Mol. Biol. (1999), 289, 123-138, PubMed 10339411 , DOI 10.1006/jmbi.1999.2740 , Abstract

Show more 1 citaion

Citation 2

AURELIA, a program for computer-aided analysis of multidimensional NMR spectra
J. Biomol. NMR (1995), 6, 255-270, PubMed 22910849 , DOI 10.1007/BF00197807 , Abstract

Hide non-primary 1 citaion

Related entities 1. HIV-1 Nef, : 1 : 6 : 48 entities Detail

Interaction partners 1. HIV-1 Nef, : 1 interactors Detail

Experiments performed 3 experiments Detail