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BMRB: 4959

1INZ

Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin

Authors

Koshiba, S., Kigawa, T., Kikuchi, A., Yokoyama, S.

Assembly

epsin ENTH domain

Entity

1. epsin ENTH domain (polymer, Thiol state: all free), 148 monomers, 17281.41 Da Detail

GSSRMSTSSL RRQMKNIVHN YSEAEIKVRE ATSNDPWGPS SSLMSEIADL TYNVVAFSEI MSMIWKRLND HGKNWRHVYK AMTLMEYLIK TGSERVSQQC KENMYAVQTL KDFQYVDRDG KDQGVNVREK AKQLVALLRD EDRLREER

Formula weight

17281.41 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 86.4 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.4 % (1521 of 1761)	83.4 % (773 of 927)	90.6 % (607 of 670)	86.0 % (141 of 164)
Backbone	93.7 % (828 of 884)	93.7 % (281 of 300)	93.6 % (410 of 438)	93.8 % (137 of 146)
Sidechain	81.1 % (826 of 1019)	78.5 % (492 of 627)	88.2 % (330 of 374)	22.2 % (4 of 18)
Aromatic	89.7 % (104 of 116)	89.7 % (52 of 58)	89.1 % (49 of 55)	100.0 % (3 of 3)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. epsin ENTH domain

GSSRMSTSSL RRQMKNIVHN YSEAEIKVRE ATSNDPWGPS SSLMSEIADL TYNVVAFSEI MSMIWKRLND HGKNWRHVYK AMTLMEYLIK TGSERVSQQC KENMYAVQTL KDFQYVDRDG KDQGVNVREK AKQLVALLRD EDRLREER

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.713 ppm	internal	direct	0.0
¹⁵N	DSS	proton	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	proton	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.713 ppm	internal	direct	0.0
¹⁵N	DSS	proton	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	proton	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.713 ppm	internal	direct	0.0
¹⁵N	DSS	proton	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	proton	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.713 ppm	internal	direct	0.0
¹⁵N	DSS	proton	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	proton	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.02 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1INZ, Strand ID: A Detail

Release date

2001-05-14

Citation

Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin
Koshiba, S., Kigawa, T., Kikuchi, A., Yokoyama, S.
J. Struct. Funct. Genomics (2002), 2, 1-8, PubMed 12836669 , Abstract

Related entities 1. epsin ENTH domain, : 1 : 9 : 106 entities Detail

Interaction partners 1. epsin ENTH domain, : 9 interactors Detail

More details for 9 interactors identified by 5 citations

Experiments performed 19 experiments Detail

1 2D 15N HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

2 3D 15N NOESY-HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

3 3D 15N TOCSY-HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

4 3D HNHB

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

5 2D 13C HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

6 3D HNCA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

7 3D HN(CO)CA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

8 3D HNCO

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

9 3D HCACO

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

10 3D CBCANH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

11 3D CBCA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

12 3D HBHA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

13 3D C(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

14 3D H(CCO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

15 3D HCCH-TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

16 3D HCCH-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

17 3D 13C NOESY-HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

18 4D 13C/15N NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

19 4D 13C/13C NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
1	epsin ENTH domain	[U-13C; U-15N]	1.0 mM
2	sodium phosphate		20 mM
3	sodium chloride		400 mM
4	dithiothreitol	[U-2H]	2 mM
5	sodium azide		0.01 %
6	H2O		90 %
7	D2O		10 %

Sample #2

Temperature 303 (±0.5) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Concentration
8	epsin ENTH domain	[U-15N]	1.0 mM
9	sodium phosphate		20 mM
10	sodium chloride		400 mM
11	dithiothreitol	[U-2H]	2 mM
12	sodium azide		0.01 %
13	H2O		90 %
14	D2O		10 %

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Found 1 Document (1.739 seconds)

BMRB: 4959

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. epsin ENTH domain, : 1 : 9 : 106 entities Detail

Interaction partners 1. epsin ENTH domain, : 9 interactors Detail

Experiments performed 19 experiments Detail

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