1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease
MKESAAAKFE RQHMDSGNSP SSSSNYCNLM MCCRKMTQGK CKPVNTFVHE SLADVKAVCS QKKVTCKDGQ TNCYQSKSTM RITDCRETGS SKYPNCAYKT TQVEKHIIVA CGGKPSVPVH FDASV
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS32:SG | 2:GSH1:SG2 |
2 | disulfide | sing | 1:CYS33:SG | 2:GSH1:SG2 |
3 | disulfide | sing | 1:CYS27:SG | 1:CYS85:SG |
4 | disulfide | sing | 1:CYS41:SG | 1:CYS96:SG |
5 | disulfide | sing | 1:CYS59:SG | 1:CYS111:SG |
6 | disulfide | sing | 1:CYS66:SG | 1:CYS73:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 86.4 % (747 of 865) | 85.0 % (623 of 733) | 93.9 % (124 of 132) |
Backbone | 97.0 % (360 of 371) | 97.2 % (244 of 251) | 96.7 % (116 of 120) |
Sidechain | 78.5 % (388 of 494) | 78.8 % (380 of 482) | 66.7 % (8 of 12) |
Aromatic | 94.9 % (37 of 39) | 94.9 % (37 of 39) | |
Methyl | 100.0 % (47 of 47) | 100.0 % (47 of 47) |
1. bovine seminal ribonuclease monomer
MKESAAAKFE RQHMDSGNSP SSSSNYCNLM MCCRKMTQGK CKPVNTFVHE SLADVKAVCS QKKVTCKDGQ TNCYQSKSTM RITDCRETGS SKYPNCAYKT TQVEKHIIVA CGGKPSVPVH FDASVTemperature 300 (±0.2) K, pH 5.65 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | bovine seminal ribonuclease monomer | [U-97% 15N] | 2.0 mM | |
2 | GLUTATHIONE | 4.0 mM |