BMRB: 4984

The Solution Structure and Interactions of CheW from Thermotoga maritima

Authors

Griswold, I.J., Zhou, H., Matison, M., Swanson, R.V., McIntosh, L.P., Simon, M.I., Dahlquist, F.W.

Assembly

CheW

Entity

1. CheW (polymer, Thiol state: not present), 151 monomers, 16954.63 Da Detail

MKTLADALKE FEVLSFEIDE QALAFDVDNI EMVIEKSDIT PVPKSRHFVE GVINLRGRII PVVNLAKILG ISFDEQKMKS IIVARTKDVE VGFLVDRVLG VLRITENQLD LTNVSDKFGK KSKGLVKTDG RLIIYLDIDK IIEEITVKEG V

Formula weight

16954.63 Da

Source organism

Thermotoga maritima

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 84.7 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.7 % (1532 of 1808)	80.2 % (752 of 938)	88.9 % (635 of 714)	92.9 % (145 of 156)
Backbone	96.1 % (865 of 900)	96.1 % (296 of 308)	95.5 % (424 of 444)	98.0 % (145 of 148)
Sidechain	76.3 % (801 of 1050)	71.9 % (453 of 630)	84.5 % (348 of 412)	0.0 % (0 of 8)
Aromatic	45.1 % (37 of 82)	43.9 % (18 of 41)	46.3 % (19 of 41)
Methyl	93.0 % (214 of 230)	93.9 % (108 of 115)	92.2 % (106 of 115)

1. CheW

MKTLADALKE FEVLSFEIDE QALAFDVDNI EMVIEKSDIT PVPKSRHFVE GVINLRGRII PVVNLAKILG ISFDEQKMKS IIVARTKDVE VGFLVDRVLG VLRITENQLD LTNVSDKFGK KSKGLVKTDG RLIIYLDIDK IIEEITVKEG V

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CheW	[U-15N]		1.0 mM

Sample #2

Temperature 308 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	CheW	[U-13C]		1.0 mM

Sample #3

Temperature 308 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	CheW	[U-15N; U-13C]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2002-04-03

Citation

The solution structure and interactions of CheW from Thermotoga maritima
Griswold, I.J., Zhou, H., Matison, M., Swanson, R.V., McIntosh, L.P., Simon, M.I., Dahlquist, F.W.
Nat. Struct. Biol. (2002), 9, 121-125, PubMed 11799399 , DOI 10.1038/nsb753 , Abstract

Related entities 1. CheW, : 3 : 4 : 16 entities Detail

Interaction partners 1. CheW, : 2 interactors Detail