BMRB: 4989

Solution Structure of B.subtilis Acyl Carrier Protein

Authors

Xu, G., Tam, A., Lin, L., Hixon, J., Fritz, C.C., Powers, R.

Assembly

B. subtilis ACYL CARRIER PROTEIN

Entity

1. B. subtilis ACYL CARRIER PROTEIN (polymer, Thiol state: not present), 81 monomers, 8871.648 Da Detail

GPLGSADTLE RVTKIIVDRL GVDEADVKLE ASFKEDLGAD SLDVVELVME LEDEFDMEIS DEDAEKIATV GDAVNYIQNQ Q

Formula weight

8871.648 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

Distance geometry, Simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.5 %, Completeness (bb): 84.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.5 % (795 of 888)	98.0 % (446 of 455)	76.1 % (265 of 348)	98.8 % (84 of 85)
Backbone	84.1 % (407 of 484)	98.2 % (163 of 166)	69.3 % (165 of 238)	98.8 % (79 of 80)
Sidechain	96.5 % (463 of 480)	97.9 % (283 of 289)	94.1 % (175 of 186)	100.0 % (5 of 5)
Aromatic	64.3 % (18 of 28)	85.7 % (12 of 14)	42.9 % (6 of 14)
Methyl	98.1 % (106 of 108)	100.0 % (54 of 54)	96.3 % (52 of 54)

1. B. subtilis ACYL CARRIER PROTEIN

GPLGSADTLE RVTKIIVDRL GVDEADVKLE ASFKEDLGAD SLDVVELVME LEDEFDMEIS DEDAEKIATV GDAVNYIQNQ Q

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Sample

Pressure 1 atm, Temperature 298 (±0) K, pH 6.4 (±0), Details The sample is the mixture of U-15N/13C labeled holo- (60%)and apo-ACP (40%)

#	Name	Isotope labeling	Type	Concentration
1	B. subtilis ACYL CARRIER PROTEIN	[U-15N; U-13C]		1 mM

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1HY8, Strand ID: A Detail

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Release date

2002-04-02

Citation

Solution structure of B. subtilis acyl carrier protein
Xu, G., Tam, A., Lin, L., Hixon, J., Fritz, C.C., Powers, R.
Structure (2001), 9, 277-287, PubMed 11525165 , DOI 10.1016/s0969-2126(01)00586-x , Abstract

Related entities 1. B. subtilis ACYL CARRIER PROTEIN, : 1 : 3 : 252 entities Detail

Interaction partners 1. B. subtilis ACYL CARRIER PROTEIN, : 2 interactors Detail

Experiments performed 1 experiments Detail