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BMRB: 4995

1IHQ

Solution NMR Structure and Folding Dynamics of the N-terminus of a rat Non-muscle Alpha-tropomyosin in an Engineered Chimeric Protein

Authors

Greenfield, N.J., Huang, Y.JANET., Palm, T., Swapna, G., Monleon, D., Montelione, G.T., Hitchcock-DeGregori, S.E.

Assembly

GlyTM1bZip coiled-coil dimer

Entity

1. GlyTM1bZip coiled-coil dimer (polymer, Thiol state: not present), 38 monomers, 4322.841 × 2 Da Detail

GAGSSSLEAV RRKIRSLQEQ NYHLENEVAR LKKLVGER

Total weight

8645.682 Da

Max. entity weight

4322.841 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

1156 CONFORMATIONALLY-RESTRICTING NOE CONSTRAINTS (OUT OF A TOTAL OF 1746 NOE DERIVED DISTANCES)

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (433 of 447)	97.5 % (232 of 238)	95.8 % (160 of 167)	97.6 % (41 of 42)
Backbone	99.1 % (226 of 228)	98.7 % (78 of 79)	100.0 % (111 of 111)	97.4 % (37 of 38)
Sidechain	95.3 % (242 of 254)	96.9 % (154 of 159)	92.3 % (84 of 91)	100.0 % (4 of 4)
Aromatic	50.0 % (6 of 12)	100.0 % (6 of 6)	0.0 % (0 of 6)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. GlyTM1bZip

GAGSSSLEAV RRKIRSLQEQ NYHLENEVAR LKKLVGER

Show sample condition

Sample

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 1IHQ, Strand ID: A, B Detail

Coupling constant

34 J values in 1 lists
Temperature 281 (±0.2) K, pH 6.4 (±0.1) Detail

Release date

2001-10-16

Citation

Solution NMR structure and folding dynamics of the N terminus of a rat non-muscle alpha-tropomyosin in an engineered chimeric protein
Greenfield, N.J., Huang, Y.JANET., Palm, T., Swapna, G., Monleon, D., Montelione, G.T., Hitchcock-DeGregori, S.E.
J. Mol. Biol. (2001), 312, 833-847, PubMed 11575936 , DOI 10.1006/jmbi.2001.4982 , Abstract

Related entities 1. GlyTM1bZip coiled-coil dimer, : 1 : 1 : 1 : 89 entities Detail

Experiments performed 20 experiments Detail

1 15N-1H HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

2 13C-!H HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

3 TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

4 NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

5 2QF-COSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

6 HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

7 HN(CA)CO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

8 CANH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

9 H(CA)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

10 H(CA)(CO)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

11 (CA)(C0)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

12 CBCANH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

13 CNCA(CO)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

14 H_HCC(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

15 C_HCC(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

16 HCCH-COSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

17 15N edited PFG NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

18 13C edited PFG NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

19 13C X-filtered NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

20 13C-15N X-filtered NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Concentration
1	GlyTM1bZip coiled-coil dimer	0.6 mM
2	NaCl	100 mM
3	sodium phosphate	10 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	GlyTM1bZip coiled-coil dimer	[U-99% 15N]	0.5 ~ 2.0 mM
7	NaCl		100 mM
8	sodium phosphate		10 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
11	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.8 mM
12	NaCl		100 mM
13	sodium phosphate		10 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Temperature 281 (±0.2) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
16	GlyTM1bZip coiled-coil dimer		1.32 mM
17	GlyTM1bZip coiled-coil dimer	[U-99% 13C; U-99% 15N]	0.88 mM
18	NaCl		100 mM
19	sodium phosphate		10 mM
20	H2O		90 %
21	D2O		10 %

nullKeywords tropomyosin, GCN4, coiled coil, triple-resonance, AutoAssign, AutoStructure, alpha-helix, automatic-assignment, folding-intermediate, isoforms

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Found 1 Document (3.401 seconds)

BMRB: 4995

Sample

Related entities 1. GlyTM1bZip coiled-coil dimer, : 1 : 1 : 1 : 89 entities Detail

Experiments performed 20 experiments Detail

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